Animal Biotechnology Laboratory, Biotechnology and Agriculture Division, Biotechnology Research Center (C.R.Bt), Ali Mendjeli, New city UV 03, BP E73, Constantine, Algeria.
Food Biotechnology Division, Biotechnology Research Center (C.R.Bt), Ali Mendjeli, New city UV 03, BP E73, Constantine, Algeria.
Acta Parasitol. 2022 Sep;67(3):1393-1400. doi: 10.1007/s11686-022-00595-5. Epub 2022 Jul 22.
This study aimed to develop a rapid spectrophotometric method for counting coccidian oocysts in broilers feces, based on a standard count method "Malassez cell".
Therefore, a raw suspension containing purified oocysts of Eimeria known to parasitize broiler chicken was used. Five concentrations of oocyst suspensions were prepared, and three series of counts using the proposed method were compared with the conventional protocol using Malassez cell to quantify oocysts. Calibration curves were drawn to correlate the specific absorbance measurement at 550 nm and the number of coccidian oocysts quantified using Malassez cell counting (oocysts mL).
The obtained results allowed to establish a formula for an easy estimation of the cell count based on their respective absorbance. The developed method can be used not only for the in vitro evaluation of the anticoccidian activity of natural or synthetic substances, but also for assessing oocysts production and the level of coccidian infection in broilers.
The estimation formula has been found to be satisfactory and useful for a wide range of Eimeria oocyst suspension, offering a methodology with high potential for automation in anticoccidian tests in vivo and in vitro.
本研究旨在基于标准计数方法“Mallasez 细胞”，开发一种用于计数肉鸡粪便中球虫卵囊的快速分光光度法。
因此，使用了含有已知寄生肉鸡的艾美耳球虫纯化卵囊的原始混悬液。制备了五个浓度的卵囊混悬液，并将使用提议方法的三组计数与使用 Malassez 细胞定量卵囊的常规方案进行了比较。绘制校准曲线以关联 550nm 处的特定吸光度测量值和使用 Malassez 细胞计数（卵囊 mL）定量的球虫卵囊数量。
获得的结果允许根据各自的吸光度建立一种用于细胞计数的简单估计公式。所开发的方法不仅可用于天然或合成物质的抗球虫活性的体外评估，还可用于评估肉鸡中卵囊的产生和球虫感染水平。
已发现该估计公式对于广泛的艾美耳球虫卵囊悬液是令人满意和有用的，为体内和体外抗球虫试验提供了一种具有高自动化潜力的方法。
