Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Antimicrob Agents. 2011 May;37(5):457-61. doi: 10.1016/j.ijantimicag.2010.12.001. Epub 2011 Feb 4.
In this study, 183 Salmonella enterica isolates were characterised for integrons and virulence genes. Among the isolates, 46% were positive for intI1, but no isolates carried intI2 or intI3. Eighteen class 1 integrons (21%) contained resistance gene cassettes (i.e. dfrA1-orfC, dfrA12-aadA2, bla(PSE-1) and aadA2) and five class 1 integrons with the dfrA12-aadA2 array were conjugally transferable. Two Salmonella pork isolates of serotypes Albany and Kedougou possessed Salmonella genomic island 1 variants SGI1-G and SGI1-F, respectively. Four class 1 integrons contained an atypical 3'-CS linked to the qacH-sul3 domain, and three were not a sul type. Two novel GyrA mutations (Pro-45→Ser and Met-48→Ile) and three novel ParC mutations (Ser-5→Arg, Thr-31→Met and Leu-77→Arg) were identified in ciprofloxacin-resistant isolates. At least 90% of the Salmonella isolates contained pagC, prgH, sitC, sipB or spaN, whereas all isolates harboured invA, msgA, spiA and tolC.
在这项研究中，对 183 株沙门氏菌分离株进行了整合子和毒力基因特征分析。在这些分离株中，46%为 intI1 阳性，但没有分离株携带 intI2 或 intI3。18 个类 1 整合子（21%）含有耐药基因盒（即 dfrA1-orfC、dfrA12-aadA2、bla(PSE-1)和 aadA2），且 5 个带有 dfrA12-aadA2 数组的类 1 整合子可通过共轭转移。2 株血清型为 Albany 和 Kedougou 的猪源沙门氏菌分离株分别携带沙门氏菌基因组岛 1 变体 SGI1-G 和 SGI1-F。4 个类 1 整合子含有与 qacH-sul3 结构域相连的非典型 3'-CS，且 3 个不属于 sul 型。在环丙沙星耐药分离株中发现了 2 个新的 GyrA 突变（Pro-45→Ser 和 Met-48→Ile）和 3 个新的 ParC 突变（Ser-5→Arg、Thr-31→Met 和 Leu-77→Arg）。至少 90%的沙门氏菌分离株含有 pagC、prgH、sitC、sipB 或 spaN，而所有分离株均携带 invA、msgA、spiA 和 tolC。
