Plasmid profile analysis of and isolated from pigs, pork and humans.

This study aimed to determine the epidemiology and association of antimicrobial resistance (AMR) among and in Thailand. The ( = 1047) and ( = 816) isolates from pigs, pork and humans were screened for 18 replicons including HI1, HI2, I1-, X, L/M, N, FIA, FIB, W, Y, P, FIC, A/C, T, FIIAs, F, K and B/O using polymerase chain reaction-based replicon typing. The ( = 26) and ( = 3) isolates carrying IncF family replicons, ESBL and/or genes were determined for FAB formula. IncF represented the major type of plasmids. Sixteen and eleven Inc groups were identified in (85.3%) and (25.7%), respectively. The predominant replicon patterns between and were IncK-F (23.7%) and IncF (46.2%). Significant correlations ( < 0.05) were observed between plasmid-replicon type and resistance phenotype. Plasmid replicon types were significantly different among sources of isolates and sampling periods. The most common FAB types between and were F2:A-:B- (30.8%) and S1:A-:B- (66.7%), respectively. In conclusion, various plasmids present in and . Responsible and prudent use of antimicrobials is suggested to reduce the selective pressures that favour the spread of AMR determinants. Further studies to understand the evolution of R plasmids and their contribution to the dissemination of AMR genes are warranted.