VU University Amsterdam, Institute of Ecological Science, Department of Animal Ecology, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
Environ Pollut. 2011 May;159(5):1343-7. doi: 10.1016/j.envpol.2011.01.014. Epub 2011 Feb 5.
The soil-living springtail Folsomia candida is frequently used in reproduction bioassays to assess soil contamination. Alternatively, the response of genes to contamination is assessed. In this study the expression of F. candida's gene encoding the deduced metallothionein-like motif containing protein (MTC) was assessed, using quantitative PCR, in response to six different metals, each at two concentrations in soil. The expression of mtc was induced after exposure to all metals, except for one chromium concentration. Exposure to soil originating from metal-contaminated field sites also induced mtc, while the expression did not change in response to a polycyclic aromatic hydrocarbon. Since this transcript is induced by most of the tested metals, it may potentially be a good indicator of metal contamination. The presented gene expression assay might become a useful tool to screen potentially polluted sites, in order to identify the ones that need further ecotoxicological investigation.
土壤生跳虫 Folsomia candida 常用于评估土壤污染的生殖生物测定中。或者，也可以评估基因对污染的反应。在这项研究中，使用定量 PCR 评估了 F. candida 编码含金属硫蛋白样基序的推断蛋白 (MTC) 的基因的表达，该基因对土壤中两种浓度的六种不同金属的反应。除了一种铬浓度外，暴露于所有金属后，mtc 的表达均被诱导。暴露于源自金属污染田间地点的土壤也诱导了 mtc 的表达，而多环芳烃则没有引起表达变化。由于这种转录物被大多数测试的金属诱导，因此它可能是金属污染的良好指示物。该基因表达测定方法可能成为筛选潜在污染场所的有用工具，以便确定需要进一步进行生态毒理学调查的场所。
