局部阿奇霉素对角膜固有免疫反应的影响。
Effect of topical azithromycin on corneal innate immune responses.
机构信息
Schepens Eye Research Institute, Boston, MA 02114, USA.
出版信息
Invest Ophthalmol Vis Sci. 2011 Apr 19;52(5):2525-31. doi: 10.1167/iovs.10-5658.
PURPOSE
To determine the effect of azithromycin (AZM) in a murine model of corneal inflammation.
METHODS
The effect of topical AZM was studied in murine corneal inflammation. Corneal inflammation was induced by thermal cautery in BALB/c mice. Leukocyte infiltration at different time points was analyzed by flow cytometry. At set time points, real-time polymerase chain reaction was performed to quantify the expression of different inflammatory cytokine transcript in the cornea. Corneal samples were analyzed immunohistochemically for the expression of intercellular adhesion molecule-1 (ICAM-1). Corneal neovascularization (CNV) was induced by micropellet (VEGF-A) placement. Mice were then treated topically with either AZM or vehicle. CNV was evaluated morphometrically.
RESULTS
Eyes receiving AZM showed a significant decrease in corneal infiltration compared with the vehicle-treated group. AZM also significantly decreased messenger RNA expression levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and ICAM-1 in the cornea. There was no significant difference in CNV between the AZM- and vehicle-treated groups.
CONCLUSIONS
After an inflammatory insult, topical AZM significantly reduced leukocyte infiltration into the cornea. This was further supported by an associated decrease in expression of IL-1β, TNF-α, and ICAM-1 in the cornea, indicating AZM may have a potential anti-inflammatory effect on corneal inflammation.
目的
在角膜炎症的小鼠模型中确定阿奇霉素（AZM）的作用。
方法
研究了局部 AZM 在小鼠角膜炎症中的作用。通过 BALB/c 小鼠的热烙术诱导角膜炎症。通过流式细胞术分析不同时间点的白细胞浸润。在设定的时间点，通过实时聚合酶链反应定量分析角膜中不同炎症细胞因子转录本的表达。通过免疫组织化学分析细胞间黏附分子-1（ICAM-1）在角膜中的表达。通过微珠（VEGF-A）放置诱导角膜新生血管化（CNV）。然后用 AZM 或载体局部治疗小鼠。通过形态计量学评估 CNV。
结果
与载体处理组相比，接受 AZM 的眼睛显示出角膜浸润明显减少。AZM 还显著降低了角膜中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和 ICAM-1 的信使 RNA 表达水平。AZM 处理组和载体处理组之间的 CNV 没有显著差异。
结论
在炎症损伤后，局部 AZM 可显著减少角膜中的白细胞浸润。这进一步得到了角膜中 IL-1β、TNF-α 和 ICAM-1 表达的相关降低的支持，表明 AZM 可能对角膜炎症具有潜在的抗炎作用。
Zotero 插件