用于产甲烷八叠球菌的穿梭载体系统，可提高转化效率。
Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency.
机构信息
Department of Biology (Area 5), University of York, Wentworth Way, York YO10 5DD, United Kingdom.
出版信息
Appl Environ Microbiol. 2011 Apr;77(7):2549-51. doi: 10.1128/AEM.02919-10. Epub 2011 Feb 4.
Abstract
We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increase in transformation efficiency for pURB500-based vectors.
摘要
我们已经鉴定出一个可读框和 DNA 元件，它们足以维持 Methanococcus maripaludis 中的穿梭载体。含有 pURB500 的 ORF1 整合到 M. maripaludis 基因组中的 S0001 菌株，支持更小的穿梭载体 pAW42，并将基于 pURB500 的载体的转化效率提高了 7000 倍。
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