从马氏甲烷球菌的串联重复序列中回收整合穿梭载体。
Recovery of an integration shuttle vector from tandem repeats in Methanococcus maripaludis.
作者信息
Sandbeck K A, Leigh J A
机构信息
Department of Microbiology, University of Washington, Seattle 98195.
出版信息
Appl Environ Microbiol. 1991 Sep;57(9):2762-3. doi: 10.1128/aem.57.9.2762-2763.1991.
Abstract
Transformation of Methanococcus maripaludis by using an integration vector, pKAS102, is described. Selection and subsequent growth at high concentrations of puromycin caused pKAS102 to develop tandem repeats within the genome. As a result, total DNA isolated from the transformant could be used to recover the intact vector by direct transformation of competent Escherichia coli.
摘要
描述了使用整合载体pKAS102对沼泽甲烷球菌进行转化的方法。在高浓度嘌呤霉素下进行选择和后续培养导致pKAS102在基因组内形成串联重复序列。因此，从转化体中分离的总DNA可通过对感受态大肠杆菌的直接转化用于回收完整的载体。
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