Department of Reproductive Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
PLoS One. 2011 Jan 31;6(1):e16220. doi: 10.1371/journal.pone.0016220.
Existing in Permanent-wave solutions (PWS), thioglycolic acid (TGA) is widely used in hairdressing industry for its contribution to hair styling. However, the toxicity of TGA, especially its reproductive toxicity, gradually calls the attention of more and more researchers.
In this work, xenopus oocytes were pretreated with different concentration of TGA, and then activated by calcium ionophore A23187. During culture, the oocytes activation rates were taken note at different time after adding calcium ionophore A23187. At the end of the culture period, the nuclear status was detected under confocal microscope. In addition, some other samples were collected for Western-Blotting analysis.
TGA significantly inhibited the oocytes activation rate and pronuclear formation. It may be resulted from the inhibition of the degradation of p-ERK1, Mos and CyclinB2.
TGA inhibits in vitro parthenogenetic activation of xenopus oocytes with inhibited the degradation of proteins involved in mitogenic-activated protein kinase (MAPK) and maturation-promoting factor (MPF) pathways.
巯基乙酸（TGA）存在于烫发溶液中，因其对发型塑造的贡献而在美发行业广泛使用。然而，TGA 的毒性，特别是其生殖毒性，逐渐引起了越来越多研究人员的关注。
在这项工作中，用不同浓度的 TGA 预处理非洲爪蟾卵母细胞，然后用钙离子载体 A23187 激活。在添加钙离子载体 A23187 后的不同时间记录卵母细胞的激活率。培养期末，在共聚焦显微镜下检测核状态。此外，还收集了一些其他样本进行 Western-Blotting 分析。
TGA 显著抑制卵母细胞的激活率和原核形成。这可能是由于 MAPK 途径中的 p-ERK1、Mos 和 CyclinB2 降解受到抑制所致。
TGA 抑制了非洲爪蟾卵母细胞的体外孤雌激活，抑制了参与有丝分裂激活蛋白激酶（MAPK）和成熟促进因子（MPF）途径的蛋白质的降解。
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