通过共聚焦免疫荧光显微镜观察非洲爪蟾卵母细胞和卵中的细胞骨架。

Visualization of the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy.

作者信息

Becker Bret E, Gard David L

机构信息

Department of Biology, University of Utah, East Salt Lake City, USA.

出版信息

Methods Mol Biol. 2006;322:69-86. doi: 10.1007/978-1-59745-000-3_6.

DOI:10.1007/978-1-59745-000-3_6

PMID:16739717

Abstract

Xelopus oocytes and eggs are popular models for studying the developmental and cellular mechanisms of RNA localization, axis specification and establishment, and nuclear envelope assembly/disassembly. However, their large size and opacity hamper application of many techniques commonly used for studying cell structure and organization, including immunofluorescence and other techniques of fluorescence microscopy. In this chapter, we describe techniques and procedures that we have used to preserve, stain, and view the cytoskeleton in Xenopus oocytes and eggs by confocal immunofluorescence microscopy.

摘要

非洲爪蟾的卵母细胞和卵是研究RNA定位、轴的特化与建立以及核膜组装/拆卸的发育和细胞机制的常用模型。然而，它们的体积较大且不透明，这妨碍了许多常用于研究细胞结构和组织的技术的应用，包括免疫荧光和荧光显微镜的其他技术。在本章中，我们描述了通过共聚焦免疫荧光显微镜观察非洲爪蟾卵母细胞和卵中细胞骨架的保存、染色和观察所使用的技术和步骤。