Department of Entomology, Kansas State University, Manhattan, Kansas, United States of America.
PLoS One. 2011 Jan 31;6(1):e16158. doi: 10.1371/journal.pone.0016158.
Ticks that feed on vertebrate hosts use their salivary secretion, which contains various bioactive components, to manipulate the host's responses. The mechanisms controlling the tick salivary gland in this dynamic process are not well understood. We identified the tick D1 receptor activated by dopamine, a potent inducer of the salivary secretion of ticks. Temporal and spatial expression patterns examined by immunohistochemistry and reverse transcription polymerase chain reaction suggest that the dopamine produced in the basal cells of salivary gland acini is secreted into the lumen and activates the D1 receptors on the luminal surface of the cells lining the acini. Therefore, we propose a paracrine function of dopamine that is mediated by the D1 receptor in the salivary gland at an early phase of feeding. The molecular and pharmacological characterization of the D1 receptor in this study provides the foundation for understanding the functions of dopamine in the blood-feeding of ticks.
以脊椎动物宿主为食的蜱利用其唾液分泌物，其中含有各种生物活性成分，来操纵宿主的反应。在这个动态过程中，控制蜱唾液腺的机制还不是很清楚。我们鉴定了一种被多巴胺激活的蜱 D1 受体，多巴胺是蜱唾液分泌的强诱导剂。通过免疫组织化学和反转录聚合酶链反应检测到的时空表达模式表明，在唾液腺腺泡的基底细胞中产生的多巴胺被分泌到腔中，并激活了细胞腔表面的 D1 受体。因此，我们提出了多巴胺的旁分泌功能，它在吸血的早期阶段通过 D1 受体在唾液腺中介导。本研究中 D1 受体的分子和药理学特征为理解多巴胺在蜱吸血中的功能提供了基础。
