Institute of Biochemical Engineering, Technische Universität, Braunschweig, Germany.
Biotechnol J. 2011 Mar;6(3):306-17. doi: 10.1002/biot.201000304. Epub 2011 Feb 7.
In the present work, the bio-based production of 1,5-diaminopentane (cadaverine), an important building block for bio-polyamides, was extended to hemicellulose a non-food raw material. For this purpose, the metabolism of 1,5-diaminopentane-producing Corynebacterium glutamicum was engineered to the use of the C(5) sugar xylose. This was realized by heterologous expression of the xylA and xylB genes from Escherichia coli, mediating the conversion of xylose into xylulose 5-phosphate (an intermediate of the pentose phosphate pathway), in a defined diaminopentane-producing C. glutamicum strain, recently obtained by systems metabolic engineering. The created mutant, C. glutamicum DAP-Xyl1, exhibited efficient production of the diamine from xylose and from mixtures of xylose and glucose. Subsequently, the novel strain was tested on industrially relevant hemicellulose fractions, mainly containing xylose and glucose as carbon source. A two-step process was developed, comprising (i) enzymatic hydrolysis of hemicellulose from dried oat spelts, and (ii) biotechnological 1,5-diaminopentane production from the obtained hydrolysates with the novel C. glutamicum strain. This now opens a future avenue towards bio-based 1,5-diaminopentane and bio-polyamides thereof from non-food raw materials.
在本工作中，将 1,5-二氨基戊烷（腐胺）的生物基生产扩展到了半纤维素——一种非食用原料。为此，对生产 1,5-二氨基戊烷的谷氨酸棒杆菌的代谢进行了工程改造，使其能够利用 C(5)糖木糖。这是通过异源表达来自大肠杆菌的 xylA 和 xylB 基因来实现的，该基因介导木糖转化为木酮糖 5-磷酸（戊糖磷酸途径的中间体），在最近通过系统代谢工程获得的、已定义的生产二氨基戊烷的谷氨酸棒杆菌菌株中实现。所创建的突变体，谷氨酸棒杆菌 DAP-Xyl1，能够有效地从木糖和木糖与葡萄糖的混合物中生产出该二胺。随后，将新型菌株用于工业相关的半纤维素馏分进行测试，这些馏分主要含有木糖和葡萄糖作为碳源。开发了两步法，包括（i）干燥的燕麦罂粟半纤维素的酶水解，和（ii）用新型谷氨酸棒杆菌菌株从获得的水解物中进行生物基 1,5-二氨基戊烷和生物聚酰胺的生产。这为从非食用原料生产生物基 1,5-二氨基戊烷和生物聚酰胺开辟了一条未来的途径。
