Laboratory of Hyperbaric Medicine, Department of Anesthesia, Center of Head and Orthopedics, Copenhagen University Hospital Rigshospitalet.
Radiat Res. 2011 Jul;176(1):84-8. doi: 10.1667/RR2304.1. Epub 2011 Feb 7.
The purpose of this study was to investigate the effects of isoflurane on unstimulated and pilocarpine-stimulated parotid saliva secretion. Ten male Sprague-Dawley rats weighing 350-400 g were randomized into two groups, and the saliva flow rate and lag phase were measured at two doses of isoflurane in a crossover study design. Increasing the isoflurane concentration from 1% to 2% was associated with a 19% decrease in saliva secretion rate, and the lag to saliva secretion was increased by 155%. To clarify whether the effect of isoflurane (1.5%) on the parotid flow varied with stimulus intensity, we measured the parotid flow induced by seven different doses of pilocarpine on sham-irradiated rats and rats irradiated with single doses of 15 Gy. A maximal pilocarpine response was obtained with 1.5 mg/kg in both irradiated and sham-irradiated rats; however, the parotid flow of the irradiated rats was 50% slower than that of the sham-irradiated rats. In conclusion, 1.5% isoflurane was found to be a good compromise between proper anesthesia and isoflurane-induced inhibition of saliva secretion. Pilocarpine induces saliva secretion in a dose-dependent matter, with supra-maximal stimulation achieved using 1.5 mg/kg.
本研究旨在探讨异氟醚对未刺激和毛果芸香碱刺激的腮腺唾液分泌的影响。10 只雄性 Sprague-Dawley 大鼠，体重 350-400g，随机分为两组，采用交叉设计研究，测量两种异氟醚剂量下的唾液流速和滞后期。异氟醚浓度从 1%增加到 2%，唾液分泌率降低 19%，唾液分泌滞后期增加 155%。为了阐明异氟醚（1.5%）对腮腺流量的影响是否随刺激强度而变化，我们在假照射大鼠和单次照射 15Gy 大鼠上测量了 7 种不同剂量毛果芸香碱诱导的腮腺流量。在照射和假照射大鼠中，1.5mg/kg 的毛果芸香碱均可获得最大反应，但照射大鼠的腮腺流量比假照射大鼠慢 50%。总之，发现 1.5%异氟醚是在适当麻醉和异氟醚抑制唾液分泌之间的良好折衷。毛果芸香碱以剂量依赖性方式诱导唾液分泌，使用 1.5mg/kg 可达到超最大刺激。
