利用基因组资源鉴定人类血吸虫（日本血吸虫）中的多态微卫星。
Polymorphic microsatellites in the human bloodfluke, Schistosoma japonicum, identified using a genomic resource.
机构信息
Institute of Parasitic Diseases, Sichuan Center for Disease Control and Prevention, Chengdu, Sichuan 610041, PR China.
出版信息
Parasit Vectors. 2011 Feb 7;4:13. doi: 10.1186/1756-3305-4-13.
Abstract
Re-emergence of schistosomiasis in regions of China where control programs have ceased requires development of molecular-genetic tools to track gene flow and assess genetic diversity of Schistosoma populations. We identified many microsatellite loci in the draft genome of Schistosoma japonicum using defined search criteria and selected a subset for further analysis. From an initial panel of 50 loci, 20 new microsatellites were selected for eventual optimization and application to a panel of worms from endemic areas. All but one of the selected microsatellites contain simple tri-nucleotide repeats. Moderate to high levels of polymorphism were detected. Numbers of alleles ranged from 6 to 14 and observed heterozygosity was always >0.6. The loci reported here will facilitate high resolution population-genetic studies on schistosomes in re-emergent foci.
摘要
在中国已停止控制项目的地区，血吸虫病的再次出现需要开发分子遗传工具来追踪基因流动并评估血吸虫种群的遗传多样性。我们使用明确的搜索标准在日本血吸虫的草图基因组中鉴定了许多微卫星基因座，并选择了一部分进行进一步分析。从最初的 50 个基因座面板中，选择了 20 个新的微卫星基因座，最终进行优化并应用于来自流行地区的一组蠕虫。所选微卫星基因座中除一个外均含有简单的三核苷酸重复序列。检测到中度至高度多态性。等位基因数量从 6 到 14 不等，观察到的杂合度始终>0.6。这里报道的基因座将有助于对重新出现的焦点中的血吸虫进行高分辨率的种群遗传研究。
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