State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
J Photochem Photobiol B. 2011 Apr 4;103(1):29-34. doi: 10.1016/j.jphotobiol.2011.01.008. Epub 2011 Jan 19.
Antimicrobial photodynamic therapy is a recently developed therapeutic option that combines a non-toxic photosensitizer with harmless visible light to damage the microbial cell. Hypocrellin A (HA), a natural occurring lipid-soluble perylenequinone pigment, has gained considerable interest since its anticancer and antiviral activities have been reported. Here, we examined the antimicrobial activity of HA against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative bacteria (Escherichia coli, Salmonella typhimurium). The results indicate that HA has a photodynamic antimicrobial activity against both Gram-positive and Gram-negative bacteria when CaCl(2) or MgCl(2) was employed. A loose binding has been established between HA and the organisms. Molecular oxygen is significantly involved in the photodynamic action of HA. Furthermore, HA maintains a photodynamic activity in terms of both types I and II reactions. Our results confirm the potential of HA to be used as a photosensitizer in antimicrobial photodynamic therapy.
抗菌光动力疗法是一种新兴的治疗选择，它将无毒的光敏剂与无害的可见光结合起来，破坏微生物细胞。竹红菌素 A（HA）是一种天然存在的脂溶性邻苯醌类色素，自从其抗癌和抗病毒活性被报道以来，已经引起了相当大的兴趣。在这里，我们研究了 HA 对革兰氏阳性（金黄色葡萄球菌、枯草芽孢杆菌）和革兰氏阴性菌（大肠杆菌、鼠伤寒沙门氏菌）的抗菌活性。结果表明，当使用 CaCl(2)或 MgCl(2)时，HA 对革兰氏阳性和革兰氏阴性细菌均具有光动力抗菌活性。HA 与生物体之间建立了松散的结合。分子氧显著参与了 HA 的光动力作用。此外，HA 在 I 型和 II 型反应方面均保持光动力活性。我们的结果证实了 HA 作为光动力抗菌治疗中的光敏剂的潜力。
