金丝桃素对甲氧西林敏感和耐药金黄色葡萄球菌生物被膜的抗菌光动力活性。
Antimicrobial photodynamic activity of hypericin against methicillin-susceptible and resistant Staphylococcus aureus biofilms.
作者信息
García Isabel, Ballesta Sofía, Gilaberte Yolanda, Rezusta Antonio, Pascual Álvaro
机构信息
Department of Microbiology, School of Medicine, Universidad de Sevilla, Sevilla, Spain.
出版信息
Future Microbiol. 2015;10(3):347-56. doi: 10.2217/fmb.14.114.
AIM
To evaluate the effectiveness of the photodynamic therapy using hypericin (HYP) against both planktonic and biofilm-forming Staphylococcus aureus.
MATERIALS & METHODS: HYP photoactivity was evaluated against methicillin-susceptible and resistant S. aureus. Bacterial suspension or biofilm were preincubated with HYP and subjected to LED illumination. Viable bacteria were determined by colony counting.
RESULTS
Preincubation with HYP (5 min) plus light exposure (10 min) showed bactericidal effect against planktonic methicillin-susceptible S. aureus and methicillin-resistant S. aureus. Longer preincubation times (24 h) and time light exposure (30 min) were required to reach HYP-photoactivity against S. aureus biofilms. HYP-photoactivity was correlated to the biofilm production.
CONCLUSION
HYP could be a potential photosensitizer for the inactivation of staphylococcal biofilms forming on the surfaces accessible to visible light.
目的
评估金丝桃素（HYP）光动力疗法对浮游态和生物被膜形成态金黄色葡萄球菌的有效性。
材料与方法
评估HYP对甲氧西林敏感和耐药金黄色葡萄球菌的光活性。将细菌悬液或生物被膜与HYP预孵育，然后进行LED光照。通过菌落计数确定活菌数。
结果
HYP预孵育（5分钟）加光照（10分钟）对浮游态甲氧西林敏感金黄色葡萄球菌和甲氧西林耐药金黄色葡萄球菌均有杀菌作用。对金黄色葡萄球菌生物被膜达到HYP光活性需要更长的预孵育时间（24小时）和光照时间（30分钟）。HYP光活性与生物被膜产生相关。
结论
HYP可能是一种潜在的光敏剂，可使在可见光可及表面形成的葡萄球菌生物被膜失活。
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