单纯疱疹病毒的非结构蛋白。I. 诱导型DNA聚合酶的纯化。
Nonstructural proteins of herpes simplex virus. I. Purification of the induced DNA polymerase.
作者信息
Powell K L, Purifoy D J
出版信息
J Virol. 1977 Nov;24(2):618-26. doi: 10.1128/JVI.24.2.618-626.1977.
Abstract
Herpes simplex virus-induced DNA polymerase purified by published methods was found to be contaminated with many others proteins, including virus structural proteins. Thus, DEAE-cellulose and phosphocellulose chromatography were used in combination with affinity chromatography to purify DNA polymerase from herpes simplex virus type 1- and type 2-infected cells. The purified enzyme retained unique features of the herpesvirus-induced DNA polymerase, including a requirement for high salt concentrations for maximal activity, a sensitivity to low phosphonoacetate concentrations, and the capacity to be neutralized by rabbit antiserum to herpesvirus-infected cells. By polyacrylamide gel electrophoresis, the purified DNA polymerase was associated with a virus-induced polypeptide of about 150,000 molecular weight.
摘要
通过已发表方法纯化的单纯疱疹病毒诱导的DNA聚合酶被发现污染有许多其他蛋白质，包括病毒结构蛋白。因此，结合离子交换纤维素和磷酸纤维素色谱法与亲和色谱法，从1型和2型单纯疱疹病毒感染的细胞中纯化DNA聚合酶。纯化后的酶保留了疱疹病毒诱导的DNA聚合酶的独特特性，包括最大活性需要高盐浓度、对低浓度膦酰乙酸敏感以及能被兔抗疱疹病毒感染细胞血清中和。通过聚丙烯酰胺凝胶电泳，纯化的DNA聚合酶与一种分子量约为150,000的病毒诱导多肽相关。
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本文引用的文献
1
Inhibition of DNA polymerase from herpes simplex virus-infected wi-38 cells by phosphonoacetic Acid.
J Virol. 1975 May;15(5):1281-3. doi: 10.1128/JVI.15.5.1281-1283.1975.
2
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
3
Acquisition of thymidine kinase activity by herpes simplex-infected mouse fibroblast cells.
Biochem Biophys Res Commun. 1963 Apr 2;11:55-9. doi: 10.1016/0006-291x(63)90027-5.
4
Human gamma globulin fractionation on anion exchange cellulose columns.
J Biol Chem. 1959 Oct;234:2645-51.
5
Regulation of herpesvirus macromolecular synthesis. I. Cascade regulation of the synthesis of three groups of viral proteins.
J Virol. 1974 Jul;14(1):8-19. doi: 10.1128/JVI.14.1.8-19.1974.
6
Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates.
J Gen Virol. 1974 Feb;22(2):171-85. doi: 10.1099/0022-1317-22-2-171.
7
Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell.
J Virol. 1973 Dec;12(6):1347-65. doi: 10.1128/JVI.12.6.1347-1365.1973.
8
Characterization of herpes simplex virus-induced deoxyribonucleic acid polymerase.
J Biol Chem. 1973 Sep 25;248(18):6270-7.
9
Altered properties of deoxyribonucleic acid nucleotidyltransferase after infection of mammalian cells with herpes simplex virus.
Nature. 1966 Apr 23;210(5034):369-71. doi: 10.1038/210369a0.
10
Virus specific antigens in mammalian cells infected with herpes simplex virus.
Immunology. 1966 Oct;11(4):399-408.
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