Tabert Amy M, Griep-Raming Jens, Guymon Andrew J, Cooks R Graham
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-2084, USA.
Anal Chem. 2003 Nov 1;75(21):5656-64. doi: 10.1021/ac0346858.
A fully multiplexed cylindrical ion trap (CIT) array mass spectrometer with four parallel ion source/mass analyzer/detector channels has been built to allow simultaneous high-throughput analysis of multiple samples. A multielement external chemical ionization/electron ionization source was coupled to a parallel array of CITs each of equal size (internal radius 2.5 mm), and the signal was recorded using an array of four miniature (2-mm inner diameter) electron multipliers. Using external electron ionization, the spectra of four separate samples were recorded simultaneously in real time using a four-channel preamplifier system and a data acquisition program written using LabVIEW software. These experiments mark the first demonstration of externally generated ions being successfully trapped in a miniature CIT mass analyzer. The instrument currently provides mass/charge range of approximately m/z 50-500. Average peak width is m/z 0.3, corresponding to a resolution of 1000 at m/z 300. The four-channel mass spectrometer is housed in a single vacuum manifold and operated with a single set of control electronics. The modular design of this instrument allows scale-up to many more channels of analysis for future applications in the areas of industrial process monitoring and combinatorial analysis and in the fields of proteomics and metabolomics.
已构建了一种具有四个平行离子源/质量分析器/检测器通道的全多路复用圆柱形离子阱（CIT）阵列质谱仪，以实现多个样品的同时高通量分析。将多元素外部化学电离/电子电离源与尺寸相等（内半径2.5毫米）的CIT平行阵列相连，并使用四个微型（内径2毫米）电子倍增器阵列记录信号。使用外部电子电离，通过四通道前置放大器系统和使用LabVIEW软件编写的数据采集程序，实时同时记录四个单独样品的光谱。这些实验标志着首次证明外部产生的离子成功被捕获在微型CIT质量分析器中。该仪器目前提供的质荷比范围约为m/z 50 - 500。平均峰宽为m/z 0.3，对应于m/z 300处1000的分辨率。四通道质谱仪安装在单个真空歧管中，并使用一套控制电子设备运行。该仪器的模块化设计允许扩展到更多分析通道，以用于工业过程监测和组合分析领域以及蛋白质组学和代谢组学领域的未来应用。
