Department of Obstetrics and Gynecology, University of Michigan, L4000 Women's Hospital, 1500 E. Medical Center Drive, Ann Arbor, MI 48109-0276, USA.
J Clin Virol. 2011 Apr;50(4):342-4. doi: 10.1016/j.jcv.2011.01.002.
Persistent infection with high-risk human papillomavirus (HPV) types is necessary for the development of high-grade cervical dysplasia and cervical carcinoma. The presence of HPV DNA in the blood of cervical cancer patients has been reported; however, whether HPV DNA is detectable in the blood of patients with pre-invasive cervical disease is unclear.
The objectives of this study were to determine if HPV 16 and HPV 18 DNA could be detected in the serum of colposcopy clinic patients, and if serum HPV detection was associated with grade of cervical disease and HPV cofactors.
Samples were selected from a biorepository collected from non-pregnant, HIV-negative women ages 18-69 attending colposcopy clinics at two urban public hospitals. Cervical disease status was based on review of colposcopy, biopsy and cytology findings. Serum HPV DNA detection was conducted using a novel PCR and mass spectroscopy-based assay.
Of the 116 adequate serum samples, all (100%) were negative for HPV 16 and HPV 18. Over half (51.7%) of participants had cervical HPV 16 and/or HPV 18 infection. Nearly one-third (31.1%) had high grade, 10.3% had low grade, and 50.9% had no cervical disease. Nearly one-third (28.5%) had ever regularly smoked cigarettes, 70.7% had early onset of sexual intercourse, and 75% had ever used oral contraceptives.
In this colposcopy clinic population with a range of clinical characteristics and established HPV cofactors, HPV DNA was undetectable in their serum. Our findings suggest that serum HPV DNA detection is not a cervical cancer screening tool.
高危型人乳头瘤病毒（HPV）的持续感染是高级别宫颈发育不良和宫颈癌发展的必要条件。已经报道了宫颈癌患者血液中 HPV DNA 的存在；然而，在宫颈疾病的前驱期患者的血液中是否可检测到 HPV DNA 尚不清楚。
本研究旨在确定是否可在阴道镜检查门诊患者的血清中检测到 HPV 16 和 HPV 18 DNA，以及血清 HPV 检测是否与宫颈疾病的严重程度和 HPV 协同因素相关。
从两个城市公立医院阴道镜检查门诊收集的非妊娠、HIV 阴性的 18-69 岁女性的生物库中选择样本。宫颈疾病的状况是基于阴道镜检查、活检和细胞学检查结果的回顾性分析。使用新型 PCR 和基于质谱的检测方法进行血清 HPV DNA 检测。
在 116 个足够的血清样本中，所有（100%）均为 HPV 16 和 HPV 18 阴性。超过一半（51.7%）的参与者有宫颈 HPV 16 和/或 HPV 18 感染。近三分之一（31.1%）有高级别病变，10.3%有低级别病变，50.9%没有宫颈疾病。近三分之一（28.5%）有经常吸烟的病史，70.7%有早期性行为，75%有过口服避孕药的使用史。
在本阴道镜检查门诊人群中，有一系列的临床特征和已确定的 HPV 协同因素，他们的血清中无法检测到 HPV DNA。我们的研究结果表明，血清 HPV DNA 检测不是宫颈癌筛查工具。
