Widschwendter Andreas, Blassnig Anya, Wiedemair Annemarie, Müller-Holzner Elisabeth, Müller Hannes M, Marth Christian
Department of Obstetrics and Gynecology, Innsbruck University Hospital, Anichstrasse 35, A-6020 Innsbruck, Austria.
Cancer Lett. 2003 Dec 30;202(2):231-9. doi: 10.1016/j.canlet.2003.09.006.
Persistent infection with human papillomavirus (HPV) has been widely recognized to induce carcinoma of the uterine cervix. Viral DNA has been documented to occur as tumor DNA in the circulation of patients with primary tumors caused by viral infection. The aim of this study was to evaluate the utility of serum HPV DNA as tumor marker in cervical cancer patients. Sera taken from 94 cervical cancer patients at the date of diagnosis and follow-up serum samples from 24 patients were examined for HPV DNA using PCR enzyme immunoassay. Serum samples taken at the date of diagnosis were HPV DNA positive in 45% of all investigated samples. Sera which were HPV DNA positive at the time of diagnosis became and also remained negative after primary treatment in patients without recurrence or persistent disease. HPV DNA was positive up to 423 days before the time of clinical diagnosis of recurrence in 10 out of 13 cases (median 72 days, range 0-423 days). Serum HPV DNA seems to reflect biological activity of the tumor. Our results indicate that serum HPV DNA might be a useful additional marker for early detection of recurrence in cervical cancer patients.
人乳头瘤病毒（HPV）持续感染已被广泛认为可诱发子宫颈癌。在由病毒感染引起的原发性肿瘤患者的循环系统中，病毒DNA已被证明可作为肿瘤DNA存在。本研究的目的是评估血清HPV DNA作为宫颈癌患者肿瘤标志物的效用。采用聚合酶链反应酶免疫分析法，对94例宫颈癌患者诊断时采集的血清以及24例患者的随访血清样本进行HPV DNA检测。在所有检测样本中，45%的诊断时采集的血清样本HPV DNA呈阳性。在无复发或持续性疾病的患者中，诊断时HPV DNA呈阳性的血清在初次治疗后转为阴性并一直保持阴性。在13例病例中的10例（中位数72天，范围0 - 423天）复发临床诊断前长达423天血清HPV DNA呈阳性。血清HPV DNA似乎反映了肿瘤的生物学活性。我们的结果表明，血清HPV DNA可能是用于早期检测宫颈癌患者复发的一种有用的辅助标志物。
