Orthner C L, MacPherson J L
Transfusion. 1984 Nov-Dec;24(6):516-9. doi: 10.1046/j.1537-2995.1984.24685066815.x.
Two alternate approaches to increasing the supply of plasma suitable for the production of cryoprecipitated AHF (cryo) were evaluated. In the first, cryo was prepared from blood collected in quadruple packs from which red cells and platelets also were made. This procedure resulted in a mean reduction of starting plasma volume of about 25 percent with a concomitant decrease in factor VIII coagulant activity (FVIII:C) of cryo when compared to approximately 2000 cryos made in triple packs assayed in our laboratory during the past year. However, American Red Cross (ARC) regions using a higher yield waterbath method of thawing plasma successfully produced quadruple pack cryos with a mean potency of 109 international units per container and about 90 percent of them met federal potency requirements. This was not the case for ARC regions using the lower yield method of thawing plasma in a refrigerator. The second approach involved cryo production from plasma frozen following 15 hours of cold storage of either the separated plasma or whole blood. Cold storage of plasma resulted in small but insignificant decreases in FVIII:C potency and yield in the cryo. However, there was a 20 percent decrease in FVIII:C yield in cryo produced from frozen plasma following 15 hours of cold storage of whole blood. Again, the decrease in FVIII:C yield resulting from interim blood storage was compensated for by the use of the higher yield waterbath method.
评估了两种增加适合生产冷沉淀抗血友病因子（cryo）的血浆供应的替代方法。第一种方法是从四联袋采集的血液中制备cryo，同时也制备红细胞和血小板。与过去一年在我们实验室检测的用三联袋制备的约2000份cryo相比，该方法使起始血浆体积平均减少约25%，同时cryo的凝血因子VIII活性（FVIII:C）也随之降低。然而，美国红十字会（ARC）采用较高产量的血浆解冻水浴法的地区成功制备出了平均效价为每容器109国际单位的四联袋cryo，其中约90%符合联邦效价要求。而ARC采用较低产量的冰箱解冻血浆法的地区则并非如此。第二种方法是在分离的血浆或全血冷藏15小时后，从冷冻的血浆中制备cryo。血浆冷藏导致cryo中FVIII:C效价和产量略有下降，但不显著。然而，全血冷藏15小时后从冷冻血浆中制备的cryo中FVIII:C产量下降了20%。同样，通过使用较高产量的水浴法弥补了因血液临时储存导致的FVIII:C产量下降。
