Tsuchiya N, Hoshino K
Third Department of Anatomy, Faculty of Medicine, Kyoto University, Japan.
Nihon Geka Hokan. 1990 Mar 1;59(2):116-33.
Mithramycin binds stoichiometrically with G-C pairs of nucleotides, thus it is useful for nuclear DNA fluorocytophotometry. In the present study, relationships between clinical features and nuclear DNA ploidy values were explored in 80 gastric cancer cases. For the first time, nuclear DNA ploidy values of tumor tissues even in the same gastric cancer were found to be not always identical, when those were determined at two different sites in the same layer or at two different depths of cancer invasion. The highest DNA ploidy values greater than 9C of the original tumors were significantly more frequently observed in advanced cancer than in early cancer. The highest DNA ploidy values greater than 12 degrees C of the original tumors were significantly more frequently observed in gastric cancer cases with lymph node metastases than in those without lymph node metastases. The highest DNA ploidy values of metastatic tumors in lymph nodes were significantly greater than those of the original tumors. The cell lines with very high nuclear DNA ploidy values, however, usually do not establish new stem lines in metastatic lymph nodes. Borrmann 2 gastric cancer which was clinically diagnosed as the localized type often showed much greater nuclear DNA ploidy values and higher incidence of metastases than Borrmann 3. Therefore, it is not true that Borrmann 2 gastric cancer is less malignant than Borrmann 3. When cancer cells invaded onto the peritoneal surface, through the serosal layer, the highest nuclear DNA ploidy values are significantly reduced for unknown reasons. Therefore, it should be kept in mind that the reduction of highest nuclear DNA ploidy values in these invasive types of gastric cancer patients does not necessarily mean a good clinical sign. Nuclear DNA ploidy analysis is the effective means of cell biological diagnosis for gastric cancer.
光神霉素与核苷酸的G-C对按化学计量结合，因此可用于细胞核DNA荧光细胞光度测定。在本研究中，对80例胃癌病例的临床特征与细胞核DNA倍体值之间的关系进行了探讨。首次发现，即使是同一例胃癌，当在同一层的两个不同部位或癌浸润的两个不同深度测定肿瘤组织的细胞核DNA倍体值时，其结果并不总是相同的。与早期癌相比，进展期癌中原发性肿瘤的最高DNA倍体值大于9C的情况明显更常见。与无淋巴结转移的胃癌病例相比，有淋巴结转移的胃癌病例中原发性肿瘤的最高DNA倍体值大于12C的情况明显更常见。淋巴结转移瘤的最高DNA倍体值明显大于原发性肿瘤。然而，细胞核DNA倍体值非常高的细胞系通常不会在转移性淋巴结中建立新的干细胞系。临床诊断为局限型的Borrmann 2型胃癌通常显示出比Borrmann 3型更大的细胞核DNA倍体值和更高的转移发生率。因此，Borrmann 2型胃癌的恶性程度低于Borrmann 3型这种说法并不正确。当癌细胞通过浆膜层侵犯到腹膜表面时，最高细胞核DNA倍体值会因不明原因而显著降低。因此，应该记住，这些侵袭性类型的胃癌患者中最高细胞核DNA倍体值的降低并不一定意味着良好的临床征象。细胞核DNA倍体分析是胃癌细胞生物学诊断的有效手段。
