Sowa M, Chung Y S, Nishimura M, Yoshino H, Maekawa H, Kato Y, Umeyama K, Kawahara M
First Department of Surgery, Osaka City University Medical School, Japan.
Eur J Surg Oncol. 1989 Aug;15(4):307-15.
The expression of carbohydrate antigens specific to Span-1, CA 19.9, and SLEX in cancer tissues and nuclear DNA ploidy patterns were studied from tissue specimens of lesions excised from 137 patients with gastric cancer. The frequency of detection of each antigen was augmented with advanced invasion depth and progress in regional lymph node metastasis. In the cases which were positive for all three kinds of antigens, lymph node metastasis and lymphatic or venous invasion were detected with significantly higher frequencies than in the negative cases. The DNA histograms showed a DNA ploidy pattern of Type Ia or Ib in the cases negative for these antigens and a non-diploid or aneuploid pattern of Type II or III in many of the positive cases. These findings suggest that an immunohistological study of gastric cancer using monoclonal antibodies, combined with a nuclear DNA ploidy analysis, might be useful for understanding malignancy of the tumour.
对137例胃癌患者病变切除组织标本，研究了Span-1、CA 19.9和SLEX特异性碳水化合物抗原在癌组织中的表达及核DNA倍体模式。随着浸润深度增加和区域淋巴结转移进展，各抗原的检测频率升高。三种抗原均阳性的病例中，淋巴结转移以及淋巴管或静脉侵犯的检出频率显著高于阴性病例。DNA直方图显示，这些抗原阴性的病例呈Ia型或Ib型DNA倍体模式，而许多阳性病例呈II型或III型非二倍体或非整倍体模式。这些发现提示，利用单克隆抗体对胃癌进行免疫组织学研究并结合核DNA倍体分析，可能有助于了解肿瘤的恶性程度。
