ALM Post-Graduate Institute of Basic Medical Sciences/Endocrinology, University of Madras, Chennai, Tamil Nadu, India.
Cell Biochem Funct. 2011 Mar;29(2):87-95. doi: 10.1002/cbf.1725. Epub 2011 Feb 9.
Urokinase-type plasminogen activator (uPA) is a serine protease that is involved in cancer progression, especially invasion and metastasis including prostate cancer. uPA activation is mediated by transactivation of uPAR and epidermal growth factor receptor (EGF-R) in prostate cancer progression. Prostate cancer (PC-3) cells have highly invasive capacity and they express uPA and uPAR gene. PC-3 cells are treated with quercetin, which inhibits invasion and migration of PC-3 cells. Quercetin downregulates uPA, uPAR and EGF, EGF-R mRNA expressions. Quercetin inhibits cell survival factor β-catenin, NF-κB and also proliferative signalling molecules such as p-EGF-R, N-Ras, Raf-1, c.Fos c.Jun and p-c.Jun protein expressions. But quercetin increased p38 mitogen-activated protein kinase protein expression. Our results suggest that quercetin inhibit migration and invasion of prostate cancer cells. It shows the value for treatment of invasive and metastasis type of prostate cancer.
尿激酶型纤溶酶原激活物（uPA）是一种丝氨酸蛋白酶，参与癌症的进展，特别是包括前列腺癌在内的侵袭和转移。uPA 的激活是通过前列腺癌进展中 uPAR 和表皮生长因子受体（EGF-R）的转激活介导的。前列腺癌细胞（PC-3）具有高度侵袭性，它们表达 uPA 和 uPAR 基因。用槲皮素处理 PC-3 细胞，抑制 PC-3 细胞的侵袭和迁移。槲皮素下调 uPA、uPAR 和 EGF、EGF-R mRNA 的表达。槲皮素抑制细胞存活因子 β-连环蛋白、NF-κB 以及增殖信号分子，如 p-EGF-R、N-Ras、Raf-1、c.Fos c.Jun 和 p-c.Jun 蛋白的表达。但是，槲皮素增加了 p38 丝裂原活化蛋白激酶蛋白的表达。我们的结果表明，槲皮素抑制前列腺癌细胞的迁移和侵袭。它显示了治疗侵袭性和转移性前列腺癌的价值。
