Li Miao, Wang Hexiang, Ng Tzi Bun
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China.
Protein Pept Lett. 2011 Jun;18(6):594-600. doi: 10.2174/092986611795222803.
A 7.3-kDa antifungal peptide was purified from dried red kidney beans. The purification procedure entailed ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, followed by fast protein liquid chromatography-gel filtration on Superdex 75. The peptide was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel and CM-cellulose. It exhibited a molecular mass of 7.3 kDa in gel filtration and also in SDS-polyacrylamide gel electrophoresis, indicating that it is a single-chained protein. The N-terminal sequence of the peptide was DGVCFGGLANGDRT. The peptide exerted an antifungal action on Fusarium oxysporum with an IC₅₀ of 3.8±0.4 µM (mean±SD, n=3). It also inhibited mycelial growth in Mycosphaerella arachidicola. It suppressed growth of lymphoma MBL2 cells and leukemia L1210 cells with an IC₅₀ of 5.2±0.4 µM and 7.6±0.6 µM, respectively. HIV-1 reverse transcriptase was inhibited with an IC₅₀ of 40±3.2 µM. However, no activity was demonstrated toward other viral enzymes.
从干红豆中纯化出一种7.3 kDa的抗真菌肽。纯化过程包括在DEAE - 纤维素上进行离子交换色谱、在Affi - gel蓝胶上进行亲和色谱、在CM - 纤维素上进行离子交换色谱，随后在Superdex 75上进行快速蛋白质液相色谱 - 凝胶过滤。该肽在DEAE - 纤维素上不吸附，但在Affi - gel蓝胶和CM - 纤维素上吸附。在凝胶过滤以及SDS - 聚丙烯酰胺凝胶电泳中，它均表现出7.3 kDa的分子量，表明它是一种单链蛋白。该肽的N端序列为DGVCFGGLANGDRT。该肽对尖孢镰刀菌具有抗真菌作用，IC₅₀为3.8±0.4 µM（平均值±标准差，n = 3）。它还抑制落花生球腔菌的菌丝生长。它抑制淋巴瘤MBL2细胞和白血病L1210细胞的生长，IC₅₀分别为5.2±0.4 µM和7.6±0.6 µM。HIV - 1逆转录酶被抑制，IC₅₀为40±3.2 µM。然而，对其他病毒酶未显示出活性。
