State Key Laboratory for Agrobiotechnology, Department of Microbiology, China Agricultural University, Beijing 100193, China.
Phytomedicine. 2011 Jan 15;18(2-3):104-9. doi: 10.1016/j.phymed.2010.06.010. Epub 2010 Aug 21.
An antifungal peptide with a defensin-like sequence and exhibiting a molecular mass of 7.3kDa was purified from dried seeds of Phaseolus vulgaris 'Cloud Bean'. The isolation procedure entailed anion exchange chromatography on DEAE-cellulose, affinity chromatography an Affi-gel blue gel, cation exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. Although the antifungal peptide was unadsorbed on DEAE-cellulose, it was adsorbed on both Affi-gel blue gel and SP-Sepharose. The antifungal peptide exerted antifungal activity against Mycosphaerella arachidicola with an IC(50) value of 1.8 μM. It was also active against Fusarium oxysporum with an IC(50) value of 2.2 μM. It had no inhibitory effect on HIV-1 reverse transcriptase when tested up to 100 μM. Proliferation of L1210 mouse leukemia cells and MBL2 lymphoma cells was inhibited by the antifungal peptide with an IC(50) of 10 μM and 40 μM, respectively.
从菜豆“云豆”干种子中纯化出一种具有防御素样序列和 7.3kDa 分子量的抗真菌肽。分离步骤包括 DEAE-纤维素阴离子交换层析、Affi-gel blue 凝胶亲和层析、SP-Sepharose 阳离子交换层析和 Superdex 75 快速蛋白液相色谱凝胶过滤。虽然该抗真菌肽不被 DEAE-纤维素吸附，但它被 Affi-gel blue 凝胶和 SP-Sepharose 吸附。该抗真菌肽对枝孢菌（Mycosphaerella arachidicola）具有抗真菌活性，IC50 值为 1.8 μM。它对尖孢镰刀菌（Fusarium oxysporum）也具有活性，IC50 值为 2.2 μM。在测试高达 100 μM 时，它对 HIV-1 逆转录酶没有抑制作用。抗真菌肽对 L1210 小鼠白血病细胞和 MBL2 淋巴瘤细胞的增殖具有抑制作用，IC50 值分别为 10 μM 和 40 μM。
