Rönnberg M, Ellfolk N
Biochim Biophys Acta. 1978 Oct 11;504(1):60-6. doi: 10.1016/0005-2728(78)90006-3.
A delay of some seconds is observed in the reaction of Pseudomonas cytochrome c peroxidase if the reaction is initiated by adding the enzyme to the reaction mixture containing reduced electron donor and hydrogen peroxide. This lag phase is avoided if the enzyme is incubated with the reduced electron donor and the reaction is started by adding hydrogen peroxide. The nature of the initial delay has been studied and it is shown that the peroxidase is reduced before a steady-state rate in the peroxidatic reaction is reached. The ability of the peroxidase to accept electrons from various electron donors emphasizes its cytochrome-like properties.
如果通过将假单胞菌细胞色素c过氧化物酶添加到含有还原态电子供体和过氧化氢的反应混合物中来启动反应，会观察到该酶的反应有几秒钟的延迟。如果将该酶与还原态电子供体一起温育，然后通过添加过氧化氢来启动反应，则可以避免这种滞后阶段。已经研究了初始延迟的性质，结果表明在过氧化物酶反应达到稳态速率之前，过氧化物酶已被还原。过氧化物酶从各种电子供体接受电子的能力突出了其类似细胞色素的性质。
