Liang Li, Zhao Yan-Lin, Yue Jun, Liu Jian-Fang, Han Min, Wang Hongxiu, Xiao Heping
Shanghai Key Laboratory of Mycobacterium Tuberculosis, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
FEMS Immunol Med Microbiol. 2011 Jun;62(1):84-90. doi: 10.1111/j.1574-695X.2011.00791.x. Epub 2011 Mar 8.
Associations of interleukin-10 (IL-10) gene promoter polymorphisms and pleural tuberculosis risk remain unclear. The objective of this study was to determine IL-10 gene promoter polymorphisms at -1082, -819 and -592 sites and their protein production in pleural fluid (PF) in patients with and without pleural tuberculosis. IL-10 gene promoter polymorphisms at the -1082, -819 and -592 sites were genotyped using a SNaPshot assay. Protein levels of IL-10 in PF were measured using an enzyme-linked immunosorbent assay. There were no significant differences in the genotype and allele frequencies of IL-10 gene promoter polymorphisms at position -1082 between the pleural tuberculosis and the control groups. However, the frequency of -819 T or -592 A alleles was significantly more common in patients with pleural tuberculosis than controls. The protein levels of IL-10 in PF were statistically higher in the pleural tuberculosis group than in the control group. Moreover, the polymorphisms at the -1082, -819 and -592 sites were associated with protein levels of IL-10 in PF in the pleural tuberculosis group, while in the control group, only the polymorphism at position -1082 correlated with the protein levels. These findings support the association between IL-10 promoter polymorphisms at -819 and -592 sites and their protein production with pleural tuberculosis risk.
白细胞介素-10（IL-10）基因启动子多态性与胸膜结核风险之间的关联仍不明确。本研究的目的是确定胸膜结核患者和非胸膜结核患者在-1082、-819和-592位点的IL-10基因启动子多态性及其在胸腔积液（PF）中的蛋白产生情况。使用SNaPshot分析对-1082、-819和-592位点的IL-10基因启动子多态性进行基因分型。使用酶联免疫吸附测定法测量PF中IL-10的蛋白水平。胸膜结核组和对照组在-1082位点的IL-10基因启动子多态性的基因型和等位基因频率没有显著差异。然而，-819 T或-592 A等位基因的频率在胸膜结核患者中比对照组显著更常见。胸膜结核组PF中IL-10的蛋白水平在统计学上高于对照组。此外，-1082、-819和-592位点的多态性与胸膜结核组PF中IL-10的蛋白水平相关，而在对照组中，只有-1082位点的多态性与蛋白水平相关。这些发现支持-819和-592位点的IL-10启动子多态性及其蛋白产生与胸膜结核风险之间的关联。
