Bodega Marine Laboratory, University of California, P. O. Box 247, 94923, Bodega Bay, California, USA.
Arch Environ Contam Toxicol. 1990 Sep-Oct;19(5):680-5. doi: 10.1007/BF01183984.
Toxicity test protocols were developed for larvae of two species of marine fishes of the Pacific coast of the United States. During winter months, larvae were obtained from adult English sole (Parophrys vetulus) spawned in the laboratory. Newly-hatched, nonfeeding larvae were used in 96-hr experiments to determine their acute sensitivity to inorganic and organic toxicants. Exposure to ZnSO(4) produced a 96-hr LC(50) of 14.5 mg Zn/L. A complex and variable organic effluent (untreated bleached kraft mill effluent; BKME) yielded 96-hr LC(50)s ranging from 1.2-9.3% BKME.Larvae were obtained from eggs collected from wild and laboratory populations of the spring and summer-spawning topsmelt (Atherinops affinis). Both acute (96-hr) mortality and longer-term (7-14 days) growth responses of topsmelt larvae to BKME were determined. Acute toxicity levels (96-hr LC(50)s) ranged from 6.2-9.7% BKME. No Observed Effect Levels (NOELs) for 7 and 14 day exposures ranged from 1.0-3.0% BKME.
为美国太平洋沿岸的两种海洋鱼类的幼虫开发了毒性测试方案。在冬季,从实验室中繁殖的成年英语鲽(Parophrys vetulus)中获得幼虫。在 96 小时的实验中,使用刚孵化的、未进食的幼虫来确定它们对无机和有机毒物的急性敏感性。暴露于 ZnSO4可产生 96 小时 LC50值为 14.5 mg Zn/L。一种复杂且可变的有机废水(未经处理的漂白纸浆厂废水;BKME)产生的 96 小时 LC50值范围为 1.2-9.3% BKME。从野生和实验室繁殖的春季和夏季产卵的竹荚鱼(Atherinops affinis)的卵中获得幼虫。用 BKME 测定了竹荚鱼幼虫的急性(96 小时)死亡率和长期(7-14 天)生长反应。急性毒性水平(96 小时 LC50值)范围为 6.2-9.7% BKME。7 天和 14 天暴露的无观察效应水平(NOEL)范围为 1.0-3.0% BKME。