Organisch-Chemisches Institut and NRW Graduate School of Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstraße 40, 48149, Münster, Germany.
Org Biomol Chem. 2011 Apr 7;9(7):2403-12. doi: 10.1039/c0ob01047b. Epub 2011 Feb 14.
A series of peptides with an alternating sequence of alkoxyamine conjugated lysine and glycine residues were synthesized by classical solution phase peptide coupling. The resulting peptides containing up to eight alkoxyamine moieties were used as initiators in nitroxide-mediated polymerization (NMP) to obtain peptide-polymer conjugates with well defined linear peptide backbones and a defined number of polymeric side chains. Polymerization of styrene and N-isopropylacrylamide (NIPAM) occurred in a highly controlled fashion. Molecular weight and polydispersity index (PDI) were determined by gel permeation chromatography (GPC). Aggregation behaviour of these hybrid materials was investigated by dynamic light scattering (DLS) and atomic force microscopy (AFM). Depending on composition, number and length of the polymer side chains, the conjugates aggregate to different topologies. Whereas peptide-polystyrene conjugates may aggregate to so called honeycomb structures, peptide-poly-N-isopropylacrylamide conjugates show differentiated aggregation behaviour.
通过经典的溶液相肽偶联方法合成了一系列具有交替序列烷氧基胺偶联赖氨酸和甘氨酸残基的肽。所得的含有多达 8 个烷氧基胺部分的肽被用作氮氧自由基介导的聚合（NMP）中的引发剂，以获得具有明确线性肽主链和确定数量的聚合侧链的肽-聚合物缀合物。苯乙烯和 N-异丙基丙烯酰胺（NIPAM）的聚合以高度可控的方式发生。通过凝胶渗透色谱（GPC）测定分子量和多分散指数（PDI）。通过动态光散射（DLS）和原子力显微镜（AFM）研究了这些杂化材料的聚集行为。根据组成、聚合物侧链的数量和长度，这些缀合物会聚集到不同的拓扑结构中。肽-聚苯乙烯缀合物可以聚集到所谓的蜂窝结构，而肽-聚-N-异丙基丙烯酰胺缀合物则表现出不同的聚集行为。
