Division of Infectious Diseases and Epidemiology, Department of Large Animal Diseases with the Clinic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland.
Vet Parasitol. 2011 Jun 10;178(3-4):339-41. doi: 10.1016/j.vetpar.2011.01.039. Epub 2011 Jan 26.
In 2007 a survey on herd-level seroprevalence of Toxoplasma gondii and Neospora caninum infections in goats in Poland was carried out. Sera were collected from all 49 breeding goat herds, scattered over the entire country, with the vast majority of them located in the western, central and northern provinces. Only adult females (≥12 months of age) were included in the study. A herd was recorded as infected if at least one seropositive female was detected. In each herd, simple random sampling was applied and sample size was determined in a way, which allowed to evaluate serological status of a herd at expected individual-level seroprevalence 10% and level of confidence 95%. In total, 1060 sera were tested using two commercial indirect ELISA kits. Sera positive to N. caninum were subsequently confirmed with IFAT. The true herd-level seroprevalence was 100% for T. gondii and 9.0% for N. caninum infection. Three herds positive to T. gondii infection were randomly selected and all adult goats were tested with an ELISA. Individual-level true seroprevalence in these herds ranged from 30.2% to 100%. This is the first time that antibodies to N. caninum have been detected in goats in Poland.
2007 年，在波兰对羊群进行了弓形虫和新孢子虫感染的血清学调查。从全国范围内的所有 49 个养殖羊群中采集了血清，其中绝大多数位于西部、中部和北部省份。本研究仅纳入成年雌性（≥12 月龄）。如果至少检测到一只血清阳性的雌性，那么这个羊群就被认为是感染的。在每个羊群中，采用简单随机抽样，确定样本量的方式可以评估预期个体水平血清阳性率为 10%、置信水平为 95%的羊群的血清学状态。总共使用了两种商业间接 ELISA 试剂盒检测了 1060 份血清。对新孢子虫呈阳性的血清随后用 IFAT 进行了确认。弓形虫的真实羊群感染率为 100%，新孢子虫感染率为 9.0%。随机选择了三个感染弓形虫的羊群，并对所有成年山羊进行了 ELISA 检测。这些羊群的个体水平真实血清阳性率从 30.2%到 100%不等。这是波兰首次在山羊中检测到新孢子虫抗体。
