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罗马尼亚奶山羊弓形体和新孢子虫血清流行率。

Seroprevalence of Toxoplasma gondii and Neospora caninum in dairy goats from Romania.

机构信息

University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, Parasitology and Parasitic Diseases Department, 3-5 Mănăştur Street, Code 400372 Cluj-Napoca, Romania.

出版信息

Vet Parasitol. 2012 May 25;186(3-4):470-4. doi: 10.1016/j.vetpar.2011.11.062. Epub 2011 Nov 25.

Abstract

Little information is available about the seroprevalence of Toxoplasma gondii and Neospora caninum infections in goats in Romania and even in Europe. During 2007-2010, 735 serum samples were collected from dairy goats located in 4 historical regions (Crişana, Maramureş, Transylvania and Muntenia) of Romania. Sera were analyzed for T. gondii and N. caninum antibodies (IgG type) by enzyme-linked immunosorbent assay (ELISA) using two commercial kits (Chekit Toxotest Antibody ELISA and Chekit Neospora caninum Antibody ELISA; Idexx-Bommeli, Switzerland). Three hundred and eighty-eight out of 735 (52.8%) goats presented T. gondii antibodies and 12 out of 512 (2.3%) goats had N. caninum antibodies. The high seroprevalence of T. gondii suggests that infection with this parasite is common in dairy goats in Romania, and less common the infection with N. caninum. This is the first time that infection with N. caninum in goats has been reported in Romania and the first extended study on seroepidemiology of T. gondii.

摘要

关于罗马尼亚乃至欧洲山羊中弓形虫和新生隐球菌感染的血清流行率的信息很少。2007 年至 2010 年期间,从罗马尼亚 4 个历史地区(Crişana、Maramureş、Transylvania 和 Muntenia)的奶山羊中采集了 735 份血清样本。使用两种商业试剂盒(Chekit Toxotest 抗体 ELISA 和 Chekit Neospora caninum 抗体 ELISA;Idexx-Bommeli,瑞士)通过酶联免疫吸附试验(ELISA)分析血清中的 T. gondii 和 N. caninum 抗体(IgG 型)。735 份血清样本中有 388 份(52.8%)呈现 T. gondii 抗体,512 份中有 12 份(2.3%)呈现 N. caninum 抗体。T. gondii 的高血清流行率表明,这种寄生虫在罗马尼亚的奶山羊中普遍存在感染,而 N. caninum 的感染则较为少见。这是罗马尼亚首次报道山羊感染 N. caninum,也是首次对 T. gondii 的血清流行病学进行扩展研究。

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