Programa de Engenharia Química/COPPE, Universidade Federal do Rio de Janeiro, Cidade Universitária, 68502, Rio de Janeiro, RJ, 21941-972, Brazil.
J Ind Microbiol Biotechnol. 2011 Sep;38(9):1575-85. doi: 10.1007/s10295-011-0948-1. Epub 2011 Feb 17.
The proper determination of experimental errors in bioprocesses can be very important because experimental errors can exert a major impact on the analysis of experimental results. Despite this, the effect of experimental errors on the analysis of bioprocess data has been largely overlooked in the literature. For this reason, we performed detailed statistical analyses of experimental errors obtained during the production of lactobionic acid and sorbitol in a system utilizing as catalyst the GFOR (glucose-fructose oxidoreductase) enzyme from permeabilized cells of the bacteria Zymomonas mobilis. The magnitude of the experimental errors thus obtained were then correlated with the process operation conditions and with the composition of the culture media used for bacterial growth. It is shown that experimental errors can depend very significantly on the operation conditions and affect the interpretation of available experimental data. More specifically, in this study, experimental errors depended on the nutritional supplements added to the cultivation medium, the inoculation process, and the reaction time, which may be of fundamental importance for actual process development. The results obtained also indicate, for the first time, that GFOR activity can be affected by the composition of the medium in which cells are cultivated.
在生物工艺过程中正确确定实验误差非常重要，因为实验误差会对实验结果的分析产生重大影响。尽管如此，实验误差对生物工艺数据分析的影响在文献中基本上被忽视了。出于这个原因，我们对利用来自渗透细胞的葡萄糖-果糖氧化还原酶（GFOR）作为催化剂的系统中生产乳糖酸和山梨糖醇过程中获得的实验误差进行了详细的统计分析。然后，将如此获得的实验误差的大小与过程操作条件以及用于细菌生长的培养基的组成相关联。结果表明，实验误差可能非常显著地取决于操作条件，并影响对现有实验数据的解释。更具体地说，在这项研究中，实验误差取决于添加到培养介质中的营养补充剂、接种过程和反应时间，这对于实际的过程开发可能具有重要意义。所得结果还首次表明，GFOR 活性可能受到细胞培养介质组成的影响。
