Department of Cellular Assay, R&D Centre, Natural Remedies Pvt. Ltd, Plot No. 5B, Veerasandra Indl. Area, 19th K. M. Stone, Hosur Road, Bangalore 560100, India.
Inflammopharmacology. 2011 Aug;19(4):235-41. doi: 10.1007/s10787-011-0080-x. Epub 2011 Feb 17.
To evaluate the inhibitory property of de-glycyrrhizinated extract of Glycyrrhiza glabra root and its phytoconstituents (glabridin, isoliquiritigenin and glycyrrhizin) on LPS-induced production of pro-inflammatory mediators.
Inhibitory effect of G. glabra extract and its phytoconstituents were studied on lipopolysaccharide (LPS)-induced nitric oxide (NO), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) levels in J774A.1 murine macrophages.
G. glabra and isoliquiritigenin significantly inhibited LPS stimulated NO, IL-1 beta and IL-6 production. Glabridin showed significant inhibition of NO and IL-1 beta release, but failed to attenuate IL-6 levels at the tested concentrations. In addition, glycyrrhizin did not exhibit inhibitory response towards any of the LPS-induced pro-inflammatory mediators at the tested concentrations.
From the results we speculate that the inhibitory effect of G. glabra extract on LPS-induced pro-inflammatory mediators is influenced by glabridin and isoliquiritigenin and is not contributed by glycyrrhizin.
评估甘草根去甘草甜素提取物及其植物成分（甘草素、异甘草素和甘草甜素）对脂多糖（LPS）诱导的促炎介质产生的抑制作用。
研究了甘草提取物及其植物成分对 J774A.1 鼠巨噬细胞中脂多糖（LPS）诱导的一氧化氮（NO）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）水平的抑制作用。
甘草和异甘草素显著抑制 LPS 刺激的 NO、IL-1β和 IL-6 的产生。甘草素对 NO 和 IL-1β的释放有显著的抑制作用，但在测试浓度下未能减轻 IL-6 的水平。此外，甘草甜素在测试浓度下对任何 LPS 诱导的促炎介质均未表现出抑制反应。
根据结果推测，甘草提取物对 LPS 诱导的促炎介质的抑制作用受甘草素和异甘草素的影响，而不受甘草甜素的影响。
