Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia.
Biochem Biophys Res Commun. 2011 Mar 18;406(3):408-13. doi: 10.1016/j.bbrc.2011.02.056. Epub 2011 Feb 15.
Ovarian cancer remains a leading cause of cancer death. A comparative proteomic study was performed on normal ovarian tissue (n=5) and grade 3 ovarian tumours (n=5) to search for differentially expressed proteins. In contrast to other studies, here we extracted proteins in soluble and insoluble protein fractions using commercial kits and also utilised three medium-range IPG strips that encompassed the broad pH range of 3-10 (pH 3-6, 5-8 and 7-10). Protein fractions were compared by 2D-PAGE and MALDI-TOF/TOF-MS. Nineteen differentially expressed proteins were identified: HSP60, Grp78, CK19, EF-Tu, MRLC2, prohibitin, Stress-70 protein, TPI and tubulin α6 were up-regulated in grade 3 tumours whereas annexin A2 and A5, antithrombin-III precursor, CBR1, GSTM2, GSTM3, RALDH1, serum albumin precursor, transthyretin precursor and vimentin were found to be down-regulated in grade 3 ovarian tumours. These proteins are associated with cytoskeleton rearrangement, cell metabolism, tumour suppression function, apoptosis and induction of host response.
卵巢癌仍然是癌症死亡的主要原因。本研究对正常卵巢组织（n=5）和 3 级卵巢肿瘤（n=5）进行了比较蛋白质组学研究，以寻找差异表达的蛋白质。与其他研究不同，这里我们使用商业试剂盒提取了可溶和不可溶蛋白部分，并使用了涵盖 pH 3-10 宽范围的三个中范围 IPG 条（pH 3-6、5-8 和 7-10）。通过 2D-PAGE 和 MALDI-TOF/TOF-MS 比较蛋白部分。鉴定出 19 种差异表达的蛋白质：HSP60、Grp78、CK19、EF-Tu、MRLC2、prohibit in、Stress-70 蛋白、TPI 和微管蛋白α6 在 3 级肿瘤中上调，而 annexin A2 和 A5、抗凝血酶-III 前体、CBR1、GSTM2、GSTM3、RALDH1、血清白蛋白前体、转甲状腺素蛋白前体和波形蛋白在 3 级卵巢肿瘤中下调。这些蛋白质与细胞骨架重排、细胞代谢、肿瘤抑制功能、细胞凋亡和诱导宿主反应有关。
