Noreen Shahzadi, Akhtar Safa, Batool Tahira, Gardner Qurratulann Afza, Akhtar Muhammad Waheed
School of Biological Sciences, University of the Punjab, Lahore 54590, Pakistan.
ACS Omega. 2021 Apr 13;6(16):10506-10514. doi: 10.1021/acsomega.0c03262. eCollection 2021 Apr 27.
Ovarian cancer (OC) is the most lethal among female reproductive system malignancies. Depending upon the stage at presentation, the five year survival ratio varies from ∼92 to ∼30%. The role of biomarkers in early cancer diagnosis, including OC, is well understood. In our previous study, through an initial screening, we have analyzed eleven proteins that exhibited differential expression in OC using two-dimensional gel electrophoresis (2D-GE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometric (MALDI-TOF MS) analysis. In continuation of our previous study, the present work describes analysis of twenty more proteins that showed aberrant expression in OC. Among these, six showed consistent significant deregulation in the OC false discovery rate [FDR ≤ 0.05]. Upon MS analysis, they were identified as vimentin, tubulin beta 2C chain, tubulin alpha 1C chain, actin cytoplasmic 2, apolipoprotein A-I, and collagen alpha 2(VI) chain [peptide mass fingerprint (PMF) score ≥ 79]. One of the differentially regulated proteins, tubulin beta 2C chain, was found to be significantly (fold change, 2.5) enhanced in OC. Verification by western blot and enzyme-linked immunosorbent assay (ELISA) demonstrated that the tubulin beta 2C chain may serve as a valuable marker for OC (ANOVA < 0.0001). The assessment of the likely association of TBB2C with OC in a larger population will not only help in developing clinically useful biomarkers in the future but also improve our understanding of the progression of OC disease.
卵巢癌（OC）是女性生殖系统恶性肿瘤中致死率最高的。根据就诊时的分期，五年生存率在约92%至约30%之间变化。生物标志物在包括OC在内的早期癌症诊断中的作用已得到充分理解。在我们之前的研究中，通过初步筛选，我们使用二维凝胶电泳（2D - GE）和基质辅助激光解吸/电离飞行时间质谱（MALDI - TOF MS）分析，分析了11种在OC中表现出差异表达的蛋白质。在我们之前研究的延续中，本工作描述了另外20种在OC中表现出异常表达的蛋白质的分析。其中，六种在OC错误发现率[FDR≤0.05]中显示出一致的显著失调。经过质谱分析，它们被鉴定为波形蛋白、微管蛋白β2C链、微管蛋白α1C链、细胞质肌动蛋白2、载脂蛋白A - I和胶原蛋白α2(VI)链[肽质量指纹（PMF）评分≥79]。一种差异调节的蛋白质，微管蛋白β2C链，在OC中被发现显著增强（倍数变化，2.5）。通过蛋白质免疫印迹和酶联免疫吸附测定（ELISA）验证表明，微管蛋白β2C链可能作为OC的一种有价值的标志物（方差分析<0.0001）。在更大的人群中评估TBB2C与OC的可能关联，不仅有助于未来开发临床上有用的生物标志物，还能增进我们对OC疾病进展的理解。
