Eyer C L, Johnson W E
Eur J Pharmacol. 1978 Oct 15;51(4):423-8. doi: 10.1016/0014-2999(78)90434-x.
Dibutyryl cyclic AMP and cyclic AMP in a DMSO medium were administered to the fetal rat heart in organ culture. Changes in heart rate were observed. Dibutyryl cyclic AMP in concentrations of 5 X 10(-5) M and 1 X 10(-4) M produced significant negative chronotropism. At a concentration of 1 X 10(-3) M, cyclic AMP in a 3% or 0.5% DMSO medium produced significant negative chronotropism. These data were compared with other studies. The negative chronotropic action of cyclic AMP and its dibutyryl derivative are evidence against the involvement of cyclic AMP in positive cardiac chronotropism.
在器官培养中，将二丁酰环磷腺苷和环磷腺苷置于二甲基亚砜（DMSO）培养基中，施用于胎鼠心脏。观察心率变化。浓度为5×10⁻⁵ M和1×10⁻⁴ M的二丁酰环磷腺苷产生显著的负性变时作用。在1×10⁻³ M的浓度下，3%或0.5% DMSO培养基中的环磷腺苷产生显著的负性变时作用。这些数据与其他研究进行了比较。环磷腺苷及其二丁酰衍生物的负性变时作用证明环磷腺苷不参与心脏正性变时作用。
