Institut für Organische Chemie, Heinrich-Buff-Ring-58, 35392 Giessen, Germany.
Anal Bioanal Chem. 2011 Apr;400(1):43-50. doi: 10.1007/s00216-011-4784-y. Epub 2011 Feb 18.
An analytical procedure for the simultaneous determination in human serum of 43 common drugs of abuse and their metabolites belonging to the different chemical and toxicological classes of amphetamines, benzodiazepines, dibenzazepines, cocaine, lysergic acid diethylamide, opioids, phencyclidine, tricyclic antidepressants, and zolpidem, using 33 deuterated standards, is presented. The sample treatment was developed to be a very simple protein precipitation and filtration. All analyses were performed with a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry in positive ionization mode. All analytes were calibrated up to 550 μg/L. The limit of detection ranged from 0.6 ng/mL (EDDP) to 13.7 ng/mL (flunitrazepam). The method has been validated according to the guidelines of the Gesellschaft für Toxikologische und Forensische Chemie, using three multiple reaction mode (MRM) transitions and retention time for positive compound identification, instead of two MRMs, in anticipation of the new guidelines for January 2011.
本研究介绍了一种在人血清中同时测定 43 种常见滥用药物及其代谢物的分析方法，这些药物属于苯丙胺、苯二氮䓬、二苯并氮䓬、可卡因、麦角酸二乙基酰胺、阿片类、苯环利定、三环抗抑郁药和唑吡坦等不同化学和毒理学类别的药物。采用 33 种氘代标准品，样品处理方法非常简单，仅需进行蛋白沉淀和过滤。所有分析均采用高效液相色谱-电喷雾串联质谱在正离子模式下进行。所有分析物的校准范围均为 550μg/L。检测限范围为 0.6ng/mL（EDDP）至 13.7ng/mL（氟硝西泮）。该方法按照德国毒理学和法医化学学会的指南进行了验证，采用了三种多重反应监测（MRM）转换和保留时间进行阳性化合物鉴定，而不是传统的两种 MRM 转换，以满足 2011 年 1 月的新指南要求。
