Tesfaye Gebreyohans, Tsegaye Wondeson, Chanie Mersha, Abinet Fisseha
College of Agriculture and Veterinary Medicine, Wollo University, Dessie, Ethiopia.
Trop Anim Health Prod. 2011 Jun;43(5):1001-5. doi: 10.1007/s11250-011-9798-0. Epub 2011 Feb 18.
A cross-sectional study was carried out on bovine brucellosis in Addis Ababa dairy farms from November 2003 to April 2004. A total of 1,202 blood samples were collected from non-vaccinated, cross-bred dairy cattle. The Rose Bengal plate test (RBPT) was used as a screening test. Those serum samples reacting positively to RBPT were subjected to the complement fixation test (CFT) for confirmation. The RBPT detected 30 of 1,202 (2.5%) of the samples as brucellosis positive. The positive sera when further retested using CFT, 18 out of the 30 RBPT positive sera were confirmed to be positive. The prevalence of brucellosis based on CFT in the study area was 1.5%, and all positive sera were from female cattle. Result of the questionnaire survey revealed that percentage of 4.4% abortion and 9.5% retained fetal membranes. Abortion and retained fetal membranes were associated with Brucella antibodies (P < 0.05). A total of 153 cattle attendants and owners in the farms were interviewed, and 73.5% were found to have no knowledge of brucellosis, only 20.8% wear protective gloves during handling aborted material and 39.6% responded that they consume raw milk. Results of this study showed that prevalence of bovine brucellosis in the study area is low and a test-and-slaughter policy can be used in order to control the diseases in dairy farms of Addis Ababa.
2003年11月至2004年4月，在亚的斯亚贝巴的奶牛场对牛布鲁氏菌病进行了一项横断面研究。共从未接种疫苗的杂交奶牛中采集了1202份血样。采用玫瑰红平板试验（RBPT）作为筛查试验。对RBPT呈阳性反应的血清样本进行补体结合试验（CFT）以进行确诊。RBPT检测出1202份样本中有30份（2.5%）为布鲁氏菌病阳性。对这30份RBPT阳性血清进一步用CFT重新检测时，其中18份被确诊为阳性。基于CFT的研究区域布鲁氏菌病患病率为1.5%，所有阳性血清均来自母牛。问卷调查结果显示，流产率为4.4%，胎膜滞留率为9.5%。流产和胎膜滞留与布鲁氏菌抗体有关（P < 0.05）。共对农场中的153名养牛员和牛主进行了访谈，发现73.5%的人对布鲁氏菌病一无所知，只有20.8%的人在处理流产材料时戴防护手套，39.6%的人表示他们饮用生牛奶。本研究结果表明，研究区域牛布鲁氏菌病患病率较低，可采用检测和屠宰政策来控制亚的斯亚贝巴奶牛场的该病。
