Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, Russian Federation 410049.
Curr Microbiol. 2011 May;62(5):1554-9. doi: 10.1007/s00284-011-9896-2. Epub 2011 Feb 18.
We evaluated the ability of several strains of the rhizobacterium Paenibacillus polymyxa, differing in the yield and rheological properties of their exopolysaccharides, to form biofilms on abiotic surfaces. Of these strains, P. polymyxa 1465, giving the highest yield of extracellular polysaccharides and the highest kinematic viscosity of the culture liquid and of aqueous polysaccharide solutions, proved to be the most active in forming biofilms on hydrophobic and hydrophilic surfaces. Enzyme-linked immunosorbent assay with rabbit polyclonal antibodies developed to isolated exopolysaccharides of P. polymyxa 1465 and 92 was used to detect P. polymyxa's polysaccharidic determinants in the composition of the biofilm materials.
我们评估了几种根际细菌多粘类芽孢杆菌菌株的能力，这些菌株在其胞外多糖的产量和流变性能上存在差异，它们能够在非生物表面形成生物膜。在这些菌株中，产量最高的胞外多糖、培养液和水溶胶溶液的运动粘度最高的多粘类芽孢杆菌 1465 菌株，在疏水和亲水表面形成生物膜的活性最高。用兔多克隆抗体建立的酶联免疫吸附试验检测到多粘类芽孢杆菌 1465 和 92 的分离胞外多糖，用于检测生物膜材料组成中多粘类芽孢杆菌的多糖决定簇。
