Brazilian Society of Rheumatology, Piauí, Brazil.
Rheumatol Int. 2012 Jun;32(6):1605-9. doi: 10.1007/s00296-011-1810-5. Epub 2011 Feb 18.
High myeloperoxidase (MPO) serum levels have been shown in several inflammatory diseases, including rheumatoid arthritis (RA). However, the correlation between MPO levels and disease activity in RA patients is still controversial. The aim of the study was to determine MPO plasma levels in RA patients and to investigate potential correlations between MPO levels and disease activity and treatment. MPO plasma levels were measured by ELISA according the manufacturer's instructions. Disease activity was measured by DAS28 ESR and DAS28 CRP scores, and patients were classified into 4 groups: group 1 DAS28 < 2.6; group 2: 2.6 ≤ DAS28 ≤ 3.2; group 3: 3.2 < DAS28 ≤ 5.1 and group 4: DAS28 > 5.1. Rheumatoid factor (RF) was measured by latex agglutination test, and anti-cyclic citrullinated peptide (anti-CCP) antibodies were detected by ELISA with a commercial kit. Fifty-seven female RA patients (mean age: 46.02 ± 13.47 years, mean disease duration: 115.77 ± 99.44 months) and sixty gender- and age-paired healthy controls were included. Mean MPO plasma levels were significantly higher in patients than in controls (72.27 pM vs. 40.78 pM, P = 0.007). RF was found in 59.6% and anti-CCP in 80.7% of the RA patients. No significant difference in MPO levels was seen among the four RA disease activity groups. We did not find significant correlation between MPO levels and disease activity as measured by DAS28 score. In conclusion, we observed significantly higher MPO plasma levels in RA patients when compared to healthy controls. However, we did not find correlation between MPO plasma level and disease activity.
高髓过氧化物酶（MPO）血清水平已在几种炎症性疾病中得到证实，包括类风湿关节炎（RA）。然而，RA 患者 MPO 水平与疾病活动度之间的相关性仍存在争议。本研究旨在确定 RA 患者的 MPO 血浆水平，并探讨 MPO 水平与疾病活动度和治疗之间的潜在相关性。MPO 血浆水平通过 ELISA 根据制造商的说明进行测量。疾病活动度通过 DAS28 ESR 和 DAS28 CRP 评分测量，患者分为 4 组：组 1 DAS28 < 2.6；组 2：2.6 ≤ DAS28 ≤ 3.2；组 3：3.2 < DAS28 ≤ 5.1 和组 4：DAS28 > 5.1。类风湿因子（RF）通过乳胶凝集试验测量，抗环瓜氨酸肽（抗-CCP）抗体通过 ELISA 用商业试剂盒检测。纳入 57 名女性 RA 患者（平均年龄：46.02 ± 13.47 岁，平均病程：115.77 ± 99.44 个月）和 60 名性别和年龄匹配的健康对照者。患者的平均 MPO 血浆水平明显高于对照组（72.27 pM 比 40.78 pM，P = 0.007）。RA 患者中 RF 阳性率为 59.6%，抗-CCP 阳性率为 80.7%。四个 RA 疾病活动组之间的 MPO 水平无显著差异。我们没有发现 MPO 水平与 DAS28 评分测量的疾病活动度之间存在显著相关性。总之，与健康对照组相比，我们观察到 RA 患者的 MPO 血浆水平明显升高。然而，我们没有发现 MPO 血浆水平与疾病活动度之间存在相关性。
