Department of Pediatric Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China.
J Mater Sci Mater Med. 2011 Apr;22(4):1037-43. doi: 10.1007/s10856-011-4257-z. Epub 2011 Feb 18.
To accelerate the fabrication of thick-walled autologous tissue-engineered vascular grafts (TEVGs), a novel biotube inserting technique was developed. After 2 weeks of subcutaneous embedding in rabbits, silicone rods (diameter, 3 mm; length, 35 mm) became encapsulated in connective tissues. Single-layered biotubes were obtained after removing the silicone rods. One silicone rod encapsulated in tissues was inserted into a single-layered biotube to form two layers of autologous tubular tissues. Three layers of autologous tubular tissues were also obtained by applying the same technique. Following a 2-week re-embedding procedure, two layers or three layers of autologous tubular tissues were integrated to form two-layered or three-layered TEVGs. Both wall thickness and burst pressure of three-layered TEVGs were significantly higher than those of two-layered and single-layered TEVGs (P < 0.05). The two-layered TEVGs could be applied as small-caliber vascular grafts, and three-layered TEVGs could be applied as medium- or large-caliber vascular grafts.
为了加速厚壁自体组织工程血管移植物（TEVG）的制造，开发了一种新的生物管插入技术。在兔子的皮下植入 2 周后，硅酮棒（直径 3 毫米；长度 35 毫米）被包裹在结缔组织中。去除硅酮棒后，即可获得单层生物管。将一根包裹在组织中的硅酮棒插入单层生物管中，形成两层自体管状组织。通过相同的技术还可以获得三层自体管状组织。经过 2 周的再包埋过程，两层或三层自体管状组织融合在一起，形成双层或三层 TEVG。三层 TEVG 的壁厚和爆破压力明显高于双层和单层 TEVG（P<0.05）。双层 TEVG 可用作小口径血管移植物，三层 TEVG 可用作中或大口径血管移植物。
