Department of Ophthalmology, University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Toyama, Japan.
Jpn J Ophthalmol. 2011 Jan;55(1):16-21. doi: 10.1007/s10384-010-0903-0. Epub 2011 Feb 18.
To report the use of hyperdried cross-linked (HDCL) amniotic membrane (AM) patching with tissue adhesive as an initial therapy for corneal perforations.
Cryopreserved AM was cross-linked with 0.1% glutaraldehyde and then dried using far-infrared rays and microwaves (hyperdry method). Three eyes of three patients with corneal perforations of up to 3 mm in diameter were included in this study. They were treated with a single-layer patch of HDCL-AM applied with a tissue adhesive (2-octyl-cyanoacrylate). We also evaluated the resistance of HDCL-AM to collagenases during in vitro digestion testing.
In all three cases, the corneal perforations were repaired within 28 days (range, 17-28 days). No recurrence occurred during the follow-up period (3-6 months). In the collagenase digestion testing, the HDCL-AM did not dissolve until 48 h, whereas the cryopreserved AM completely dissolved within 60 min.
Three cases of corneal perforations were successfully managed using HDCL-AM patching with tissue adhesive. The HDCL-AM was resistant to collagenases during in vitro digestion testing. The HDCL-AM was a useful substrate for corneal perforations. This simple surgical technique may be one of the initial therapeutic options for corneal perforations.
报告使用超干燥交联（HDCL）羊膜（AM）贴片和组织粘合剂作为角膜穿孔的初始治疗方法。
将冷冻保存的 AM 用 0.1％戊二醛交联，然后使用远红外线和微波（超干燥法）干燥。本研究纳入了 3 例直径达 3mm 的角膜穿孔患者的 3 只眼。他们接受了单层 HDCL-AM 贴片治疗，并用组织粘合剂（2-辛基-氰基丙烯酸酯）固定。我们还评估了 HDCL-AM 在体外消化试验中对胶原酶的抵抗力。
在所有 3 例中，角膜穿孔均在 28 天内（17-28 天）得到修复。在随访期间（3-6 个月）未发生复发。在胶原酶消化试验中，HDCL-AM 直到 48 小时才溶解，而冷冻保存的 AM 在 60 分钟内完全溶解。
使用组织粘合剂的 HDCL-AM 贴片成功治疗了 3 例角膜穿孔。HDCL-AM 在体外消化试验中对胶原酶具有抵抗力。HDCL-AM 是治疗角膜穿孔的有用基质。这种简单的手术技术可能是角膜穿孔的初始治疗选择之一。
