Service de Biologie de la Reproduction, CHU de Dijon, France.
Syst Biol Reprod Med. 2011 Jun;57(3):139-48. doi: 10.3109/19396368.2010.549538. Epub 2011 Feb 21.
Prostasomes, vesicles present in human semen, are known to play a role in male fertility. However, the mechanisms involved are still poorly understood. The present study looks at the direct influence of different concentrations of prostasomes on human sperm function in conditions supporting capacitation in vitro. Five million Percoll selected spermatozoa were incubated for 3 h at 37°C under an atmosphere of 5% CO₂ in air, in 100 µl Biggers Whitten Whittingham's medium (BWW) containing polyvinyl alcohol (PVA, 1 mg/ml) and bovine serum albumin (BSA; 3 mg/ml) in the absence or presence of increasing concentrations of prostasomes (expressed in terms of their cholesterol content: 15; 30; 45 nmoles per 100 µL of incubation medium). After in vitro exposure of spermatozoa to prostasomes, our data indicate that i) tyrosine phosphorylation intensity of the 107 KDa protein band was dose dependently lower and ii) the percentage of viable and progressive motile spermatozoa was unchanged and the percentage of non-progressive motility decreased. In addition, the incubation of prostasomes with spermatozoa resulted in an enrichment of their lipid content. Our experiments suggest that adhesion of prostasomes to spermatozoa could be responsible for the decrease in Tyrosine phosphorylation and the alteration of the mean curvilinear velocity (VCL) and the average path velocity (VAP).
前列腺小体是存在于人类精液中的囊泡，已知其在男性生育力中发挥作用。然而，其涉及的机制仍知之甚少。本研究探讨了不同浓度的前列腺小体在体外支持获能条件下对人精子功能的直接影响。将 500 万个 Percoll 精选的精子在 37°C 下孵育 3 小时，在 5%CO₂的大气中，在含有聚维酮（PVA，1mg/ml）和牛血清白蛋白（BSA；3mg/ml）的 100µl Biggers Whitten Whittingham 培养基（BWW）中孵育，不存在或存在不同浓度的前列腺小体（以其胆固醇含量表示：15；30；45nmoles/100µL 孵育培养基）。在精子暴露于前列腺小体的体外后，我们的数据表明：i）107 KDa 蛋白带的酪氨酸磷酸化强度呈剂量依赖性降低，ii）存活和进行性运动精子的百分比不变，非进行性运动精子的百分比降低。此外，前列腺小体与精子孵育会导致其脂质含量增加。我们的实验表明，前列腺小体与精子的黏附可能是导致酪氨酸磷酸化减少以及平均曲线速度（VCL）和平均路径速度（VAP）改变的原因。
