Laboratorio de Inmunología, División de Disciplinas, Básicas, Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, México.
Oral Dis. 2011 Jul;17(5):522-9. doi: 10.1111/j.1601-0825.2011.01798.x. Epub 2011 Feb 18.
The aim of this study was to compare the levels of interleukin-12 (IL-12) and IL-18 in gingival tissue and serum between patients with chronic (n = 18) or aggressive periodontitis (n = 12) and healthy subjects (HS) (n = 9).
Gingival tissue biopsies and serum were obtained from all study subjects. The tissue was homogenized and cytokines IL-12 and IL-18 were quantified by enzyme-linked immunosorbent assay.
Interleukin-12 levels in gingival tissue were significantly higher in aggressive periodontitis patients than in HS; serum IL-12 was significantly elevated in aggressive periodontitis relative to both chronic periodontitis (CP) and HS. IL-18 levels in gingival tissue showed no significant differences between the groups. Patients with CP showed significantly elevated levels of serum IL-18 compared with HS; however, the aggressive periodontitis group showed no significant differences with either the CP group or the HS.
Our results showed higher levels of IL-12 in gingival tissue and serum of patients with aggressive periodontitis, and IL-18 was elevated in the serum of CP patients. The patterns of IL-12 and IL-18 are different in chronic and aggressive periodontitis; this finding suggests distinctive mechanisms of immunopathogenesis between these forms of periodontitis.
本研究旨在比较慢性（n=18）或侵袭性牙周炎（n=12）患者与健康受试者（HS）（n=9）的牙龈组织和血清中白细胞介素-12（IL-12）和白细胞介素-18（IL-18）水平。
从所有研究对象中获取牙龈组织活检和血清。组织匀浆后，采用酶联免疫吸附试验定量检测细胞因子 IL-12 和 IL-18。
侵袭性牙周炎患者的牙龈组织中白细胞介素-12 水平明显高于 HS；与 CP 和 HS 相比，侵袭性牙周炎患者血清中 IL-12 水平显著升高。牙龈组织中 IL-18 水平在各组间无显著差异。与 HS 相比，CP 患者血清中 IL-18 水平显著升高；然而，侵袭性牙周炎组与 CP 组或 HS 组之间均无显著差异。
我们的研究结果表明，侵袭性牙周炎患者的牙龈组织和血清中 IL-12 水平升高，CP 患者的血清中 IL-18 水平升高。IL-12 和 IL-18 的模式在慢性和侵袭性牙周炎中不同；这一发现表明这两种形式的牙周炎具有不同的免疫发病机制。
