Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, Japan.
Aust Dent J. 2011 Mar;56(1):40-7. doi: 10.1111/j.1834-7819.2010.01284.x. Epub 2011 Jan 10.
The aim of this study was to establish a new in vitro model for biofilm induced secondary caries studies using an oral biofilm reactor.
An approximately 2 × 3 × 2 mm(3) sized dentino-enamel Class I cavity was prepared in the middle of a square-shaped specimen from the mid-labial portion of bovine incisors. The cavities were partially filled with either Clearfil AP-X with SE-Bond or Clearfil AP-X without any bond. Artificial biofilms were then formed on the resin composite filled surfaces using three species of oral bacteria in an oral biofilm reactor for 20 hours followed by 7- or 30-day incubation periods.
The lesions were clearly visible on fluorescence microscopy and by scanning electron microscopy in the enamel at the interface of resin restorations in all samples. The data from image analysis showed that the lesion size was largest in the No-bond samples with statistically significant differences (p < 0.05). Demineralization along the cavity wall extended deeper in No-bond compared to SE-Bond samples and penetration was significantly deeper in No-bond 30-day samples.
A primary artificial secondary caries model was established using biofilms for in vitro studies and the significance of using a bonding system could also be verified.
本研究旨在利用口腔生物膜反应器建立用于生物膜诱导继发龋研究的新体外模型。
从牛切牙的中唇部分制备大约 2×3×2mm(3)大小的牙釉质-牙本质 I 类腔在方形标本的中间。腔部分用 Clearfil AP-X 与 SE-Bond 或 Clearfil AP-X 填充，没有任何粘结。然后，使用三种口腔细菌在口腔生物膜反应器中在树脂复合材料填充表面上形成人工生物膜，培养 20 小时，然后进行 7 天或 30 天孵育。
在所有样本中，荧光显微镜和扫描电子显微镜均清晰可见树脂修复体界面处釉质上的病变。图像分析数据显示，无粘结样本的病变大小最大，差异具有统计学意义（p<0.05）。与 SE-Bond 样本相比，无粘结样本的腔壁沿脱矿延伸更深，无粘结 30 天样本的穿透深度更深。
使用生物膜建立了用于体外研究的原发性人工继发龋模型，也验证了使用粘结系统的重要性。
