Department of Biotechnology Shivaji University, Kolhapur, India.
J Appl Microbiol. 2011 May;110(5):1235-44. doi: 10.1111/j.1365-2672.2011.04976.x. Epub 2011 Mar 14.
To optimize the medium components for the production of indole-3-acetic acid (IAA) by isolated bacterium Pantoea agglomerans strain PVM.
Present study deals with the production of an essential plant hormone IAA by a bacterial isolate P. agglomerans strain PVM identified by 16S rRNA gene sequence analysis. The medium containing 8g l(-1) of meat extract and 1g l(-1) of l-tryptophan (precursor) at optimum pH 7, 30°C and 48-h incubation gave the maximum production of IAA (2·191 g l(-1) ). Effect of IAA synthesized on in vitro root induction in Nicotiana tobacum (leaf) explants was compared with that of control. IAA was characterized by high-performance thin-layer chromatography, high-performance liquid chromatography and gas chromatography-mass spectroscopy.
Pantoea agglomerans strain PVM was a good candidate for the inexpensive and utmost production of IAA in short period, as it requires simple medium (meat extract and l-tryptophan).
The present report first time showed the rapid, cost-effective and maximum production of IAA. No reports are available on the optimization of particular medium components for the production of IAA. This study demonstrates a novel approach for in vitro root induction in N. tobacum (leaf) explants.
优化聚生肠杆菌 PVM 菌株生产吲哚-3-乙酸（IAA）的培养基成分。
本研究通过 16S rRNA 基因序列分析鉴定出一种细菌分离株聚生肠杆菌 PVM，可以产生一种必需的植物激素 IAA。在最佳 pH 值为 7、30°C 和 48 小时孵育条件下，含 8g/L 肉浸膏和 1g/L l-色氨酸（前体）的培养基可获得最大的 IAA 产量（2.191g/L）。将合成的 IAA 与对照进行比较，观察其对烟草（叶片）外植体离体生根的影响。通过高效薄层层析、高效液相色谱和气相色谱-质谱对 IAA 进行了表征。
聚生肠杆菌 PVM 是一种生产 IAA 的良好候选菌株，因为它可以在短时间内以廉价和最佳的方式生产 IAA，且所需的培养基简单（肉浸膏和 l-色氨酸）。
本报告首次展示了快速、经济高效和最大量生产 IAA 的方法。目前尚无关于优化特定培养基成分生产 IAA 的报道。本研究为烟草（叶片）外植体的离体生根提供了一种新方法。
