Batykova Zhuldyz, Pidlisnyuk Valentina, Kistaubayeva Aida, Ust'ak Sergey, Savitskaya Irina, Saidullayeva Laila, Mamirova Aigerim
Department of Biotechnology, Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty 050040, Kazakhstan.
Department of Environmental Chemistry & Technology, Faculty of Environment, Jan Evangelista Purkyně University, Pasteurova 1, 400 96 Ústí nad Labem, Czech Republic.
Microorganisms. 2025 Aug 15;13(8):1902. doi: 10.3390/microorganisms13081902.
Plant growth-promoting rhizobacteria (PGPRs) colonise the rhizosphere and root surfaces, enhancing crop development through a variety of mechanisms. This study evaluated microbial strains isolated from L. for key plant growth-promoting traits, including indole-3-acetic acid (IAA) production, phosphate and zinc (Zn) solubilisation, nitrogen (N) fixation, and antifungal activity. Among 36 isolates, 3 (AS8, AS23, AS31) exhibited strong growth-promoting potential. IAA production, citrate assimilation, carbohydrate fermentation, and catalase activity were observed to a comparable extent among the selected strains. AS8 showed the highest protease, lipase, and amylolytic activity, while AS23 demonstrated superior phosphate and Zn solubilisation. Notably, AS31 emerged as the most promising multi-trait isolate, exhibiting the highest levels of IAA production, N fixation, antifungal activity against five phytopathogens (, , , , and ), potentially linked to its hydrogen sulphide (HS) production, and cellulolytic activity. Molecular identification based on 16S rRNA gene sequencing revealed the isolates as AS8, AS23, and AS31. Seed germination assays confirmed the plant growth-promoting efficacy of these PGPR strains, with vigour index increases of up to 43.4-fold. Given their positive impact on seed germination and significant Zn-solubilising abilities, the selected strains represent promising candidates for use as bio-inoculants, offering a sustainable and eco-friendly strategy to enhance agricultural productivity in nutrient-deficient soils. Future research should validate the efficacy of these PGPR strains under pot conditions to confirm their potential for practical agricultural applications.
植物促生根际细菌（PGPRs）定殖于根际和根表面，通过多种机制促进作物生长。本研究评估了从[具体植物名称未给出]中分离出的微生物菌株的关键植物促生特性，包括吲哚 - 3 - 乙酸（IAA）的产生、磷酸盐和锌（Zn）的溶解、氮（N）固定以及抗真菌活性。在36株分离物中，有3株（AS8、AS23、AS31）表现出强大的促生潜力。在所选菌株中，IAA产生、柠檬酸盐同化、碳水化合物发酵和过氧化氢酶活性的观察程度相当。AS8表现出最高的蛋白酶、脂肪酶和淀粉酶活性，而AS23在磷酸盐和Zn溶解方面表现优异。值得注意的是，AS31成为最有前景的多性状分离物，表现出最高水平的IAA产生、N固定、对五种植物病原体（[未明确病原体名称]）的抗真菌活性，这可能与其硫化氢（HS）产生和纤维素分解活性有关。基于16S rRNA基因测序的分子鉴定表明，分离物为[具体微生物名称未给出]的AS8、AS23和AS31。种子发芽试验证实了这些PGPR菌株的植物促生功效，活力指数增加高达43.4倍。鉴于它们对种子发芽的积极影响和显著的Zn溶解能力，所选菌株是用作生物接种剂的有前途的候选者，为提高缺营养土壤中的农业生产力提供了一种可持续且环保的策略。未来的研究应在盆栽条件下验证这些PGPR菌株的功效，以确认它们在实际农业应用中的潜力。
