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RNA 标准品在评价 RT-qPCR 检测和平台中的适用性。

Applicability of RNA standards for evaluating RT-qPCR assays and platforms.

机构信息

LGC Limited, Queens Road, Teddington, Middlesex, TW11 0LY, UK.

出版信息

BMC Genomics. 2011 Feb 18;12:118. doi: 10.1186/1471-2164-12-118.

DOI:10.1186/1471-2164-12-118
PMID:21332979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3052187/
Abstract

The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm® BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.

摘要

不同的 RT-qPCR 分析方法和技术的可用性阻碍了平台间数据的可比性。需要参考标准来促进平台评估和可比性。我们已经探讨了使用通用 RNA 标准来比较新型 qPCR 平台（Fluidigm® BioMark™）与广泛使用的 ABI 7900HT 系统的性能。我们的结果表明，此类标准可以成为评估平台关键性能特征的工具包的一部分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/03324ae25f41/1471-2164-12-118-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/3f903c8bc900/1471-2164-12-118-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/96344ce456e4/1471-2164-12-118-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/3dc6e5c2baf6/1471-2164-12-118-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/03324ae25f41/1471-2164-12-118-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/3f903c8bc900/1471-2164-12-118-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/96344ce456e4/1471-2164-12-118-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/3dc6e5c2baf6/1471-2164-12-118-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049a/3052187/03324ae25f41/1471-2164-12-118-4.jpg

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