Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul, 06591, Republic of Korea.
Korea Zoonosis Research Institute, Jeonbuk National University, Iksan, 54596, Republic of Korea.
J Microbiol. 2020 Aug;58(8):711-715. doi: 10.1007/s12275-020-0109-1. Epub 2020 May 18.
Severe fever with thrombocytopenia syndrome (SFTS) is being reported annually in South Korea since its first detection there in 2010. The causal agent is a negative-strand RNA virus 80-100 nm in diameter. It causes fever, thrombocytopenia, leukocytopenia, gastrointestinal symptoms, and neural symptoms. The mortality rate of SFTS was 32.6% among 172 cases reported from 2012 to 2015 in South Korea. Thus, is necessary to develop an effective diagnostic method that selectively identifies the isolates circulating in South Korea. The real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a simple, rapid, and sensitive approach for molecular diagnosis. Here, we designed novel primers for this assay and found that the technique had very high specificity, sensitivity, and efficiency. This real-time RT-LAMP approach using the novel primers developed herein can be applied for early diagnosis of SFTSV strains in South Korea to reduce the mortality rate of SFTS.
自 2010 年在韩国首次发现以来，韩国每年都有严重发热伴血小板减少综合征（SFTS）的报告。病原体是一种直径 80-100nm 的负链 RNA 病毒。它会引起发热、血小板减少、白细胞减少、胃肠道症状和神经症状。2012 年至 2015 年，韩国报告了 172 例 SFTS 病例，死亡率为 32.6%。因此，有必要开发一种有效的诊断方法，选择性地识别在韩国流行的分离株。实时逆转录环介导等温扩增（RT-LAMP）检测是一种简单、快速、灵敏的分子诊断方法。在这里，我们为该检测设计了新型引物，发现该技术具有很高的特异性、灵敏度和效率。本研究采用新型引物的实时 RT-LAMP 方法可用于韩国 SFTSV 株的早期诊断，从而降低 SFTS 的死亡率。
