Faculty of Postgraduate Studies for Advanced Sciences, Beni-suef University, Beni-Suef 62511, Egypt.
Noto Marine Laboratory, Institute of Nature and Environmental Technology, Division of Marine Environmental Studies, Kanazawa University, Noto-cho, Ishikawa 927-0553, Japan.
Int J Environ Res Public Health. 2020 Feb 21;17(4):1391. doi: 10.3390/ijerph17041391.
It has been reported that spinal deformity was induced in developing fish by the addition of polycyclic aromatic hydrocarbons (PAHs). To examine the mechanism of the disruption of fish bone metabolism, the effect of benz[]anthracene (BaA), a kind of PAH, on plasma calcium, inorganic phosphorus, osteoblasts, and osteoclasts was investigated in this study. We also measured several plasma components to analyze the toxicity of BaA on other metabolisms. BaA (1 or 10 ng/g body weight) was intraperitoneally injected (four times) into nibbler fish during breeding, for 10 days, and it was indicated, for the first time, that injecting high doses of BaA to nibbler fish induced both hypocalcemia and hypophosphatemia. Furthermore, in the scales of nibbler fish treated with high doses of BaA, both osteoclastic and osteoblastic marker messengerRNA (mRNA) expressions decreased. These results are a cause of disruption of bone metabolism and, perhaps, the induction of spinal deformities. In addition, we found that total protein, metabolic enzymes in the liver, total cholesterol, free cholesterol, and high-density lipoprotein cholesterol levels significantly decreased in BaA-injected fish. These results indicate that BaA may affect liver diseases and emphasize the importance of prevention of aquatic PAH pollution.
据报道，多环芳烃（PAHs）的添加会导致鱼类脊柱畸形。为了研究鱼类骨骼代谢紊乱的机制，本研究考察了苯并[a]蒽（BaA）这一 PAH 对血浆钙、无机磷、成骨细胞和破骨细胞的影响。我们还测量了几种血浆成分，以分析 BaA 对其他代谢物的毒性。在繁殖期间，将 BaA（1 或 10ng/g 体重）通过腹腔注射（四次）到 nibbler 鱼体内，持续 10 天。结果首次表明，向 nibbler 鱼注射高剂量的 BaA 会导致低钙血症和低磷血症。此外，在高剂量 BaA 处理的 nibbler 鱼鳞片中，破骨细胞和成骨细胞标志物的信使 RNA（mRNA）表达均降低。这些结果是骨骼代谢紊乱的原因，可能导致脊柱畸形。此外，我们发现 BaA 注射鱼的总蛋白、肝脏代谢酶、总胆固醇、游离胆固醇和高密度脂蛋白胆固醇水平显著降低。这些结果表明 BaA 可能影响肝脏疾病，并强调了预防水相 PAH 污染的重要性。
